TY - JOUR
T1 - Purification and NH2-terminal amino acid sequencing of the β subunit of a human T-cell antigen receptor
AU - Acuto, O.
AU - Fabbi, M.
AU - Smart, J.
PY - 1984
Y1 - 1984
N2 - To obtain information about the structural basis for T-cell antigen recognition, a T3-associated Ti receptor molecule was isolated from crude membranes of the REX human thymic tumor line and purified by affinity chromatography with an anti-clonotypic monoclonal antibody in conjunction with preparative gel electrophoresis. NH2-terminal amino acid sequencing of the β subunit unambiguously identified the amino acids in positions 2-12. Comparative protein sequence analysis by computer search demonstrated that this Ti β sequence bore weak, but definite, homology to the first framework of the variable region of human λ light chain. Antisera to a synthetic peptide corresponding to positions 2-11 precipitated the denatured Ti β subunit from REX, thus confirming the above sequence. This information suggests that the Ti β subunit is distantly related to human immunoglobulin λ light chain and, moreover, should be of use in the molecular cloning of the Ti β gene.
AB - To obtain information about the structural basis for T-cell antigen recognition, a T3-associated Ti receptor molecule was isolated from crude membranes of the REX human thymic tumor line and purified by affinity chromatography with an anti-clonotypic monoclonal antibody in conjunction with preparative gel electrophoresis. NH2-terminal amino acid sequencing of the β subunit unambiguously identified the amino acids in positions 2-12. Comparative protein sequence analysis by computer search demonstrated that this Ti β sequence bore weak, but definite, homology to the first framework of the variable region of human λ light chain. Antisera to a synthetic peptide corresponding to positions 2-11 precipitated the denatured Ti β subunit from REX, thus confirming the above sequence. This information suggests that the Ti β subunit is distantly related to human immunoglobulin λ light chain and, moreover, should be of use in the molecular cloning of the Ti β gene.
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M3 - Article
C2 - 6427776
AN - SCOPUS:0021448827
VL - 81
SP - 3851
EP - 3855
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 12 I
ER -