TY - JOUR
T1 - Purification and partial characterization of a new 85 KDa amyloidosis-related protein in chronic hemodialysis
AU - Brancaccio, Diego
AU - Ghiggeri, GianMarco
AU - Garberi, Alessandro
AU - Anelli, Adriana
AU - Ginevri, Fabrizio
AU - Loggi, Guido
AU - Gusmano, Rosanna
PY - 1991/5/15
Y1 - 1991/5/15
N2 - An 85 KDa protein was purified by a multistep procedure (ultracentrifugation, HPLC, SDS-PAGE) from sera and amyloid deposits of patients on chronic hemodialysis and was characterized as a novel protein on the basis of its NH2 terminus (KVQLVE-V). This protein was formed by two subunits with Mr of 55 and 30 KDa and had affinity for Thyoflavin T, a fluorescent dye which was employed for labelling the protein prior HPLC. The 85 KDa was the only fluorescent component of ultracentrifugates from the serum of hemodialyzed patients while in amyloid fibrils it coexisted in roughly equimolar amounts with β2-microglobulin. This new high molecular weight protein which accumulates in uremia, could be co-responsible with β2-microglobulin for hemodialysis-related osteoarticular amyloidosis.
AB - An 85 KDa protein was purified by a multistep procedure (ultracentrifugation, HPLC, SDS-PAGE) from sera and amyloid deposits of patients on chronic hemodialysis and was characterized as a novel protein on the basis of its NH2 terminus (KVQLVE-V). This protein was formed by two subunits with Mr of 55 and 30 KDa and had affinity for Thyoflavin T, a fluorescent dye which was employed for labelling the protein prior HPLC. The 85 KDa was the only fluorescent component of ultracentrifugates from the serum of hemodialyzed patients while in amyloid fibrils it coexisted in roughly equimolar amounts with β2-microglobulin. This new high molecular weight protein which accumulates in uremia, could be co-responsible with β2-microglobulin for hemodialysis-related osteoarticular amyloidosis.
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U2 - 10.1016/0006-291X(91)90387-M
DO - 10.1016/0006-291X(91)90387-M
M3 - Article
C2 - 2039487
AN - SCOPUS:0025915764
VL - 176
SP - 1037
EP - 1043
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -