Carnitine palmitoyl-transferase has been extracted with 0.5% Tween-20 from human liver homogenatc and purified to homogeneity. The purified enzyme has a native Mr of 274 kDa. The subunit Mr is of 66 kDa, as shown by SDS-PAGE and immunoblots obtained with antibodies raised against human CPT. Purified CPT shows high affinity for palmitoyl-CoA and palmitoyl-carnitine and is not inhibited by malonyl-CoA, Seven tryptic peptides and the N-terminal of purified human CPT have been sequenced, and found homologous to rat CPT sequence. Both antibodies and peptide sequences are important tools for the investigation of the molecular basis of CPT deficiency in man.
- Amino acid sequence
- Carnitine palmitoyl-transferase
- Human liver
ASJC Scopus subject areas
- Molecular Biology