TY - JOUR
T1 - Purification, inhibitory properties, amino acid sequence and identification of the reactive site of a new serine proteinase inhibitor from oil-rape (itBrassica napus) seed
AU - Ceciliani, Fabrizio
AU - Bortolotti, Fabrizio
AU - Menegatti, Enea
AU - Ronchi, Severino
AU - Ascenzi, Paolo
AU - Palmieri, Sandro
PY - 1994/4/4
Y1 - 1994/4/4
N2 - A new serine proteinase inhibitor, rapeseed trypsin inhibitor (RTI), has been isolated from rapeseed (Brassica napus var. oleifera) seed. The protein inhibits the catalytic activity of bovine β-trypsin and bovine α-chymotrypsin with apparent dissociation constants of 3.0 × 10-10 M and 4.1 × 10-7 M, at pH 8.0 and 21°C, respectively. The stoichiometry of both proteinase-inhibitor complexes is 1:1. The amino acid sequence of RTI consists of 60 amino acid residues, corresponding to an Mr, of about 6.7 kDa. The p1-pi, reactive site bond has been tentatively identified at position Arg20-Ile21. RTI shows no similarity to other serine proteinase inhibitors except the low molecular weight mustard trypsin inhibitor (MTI-2). RTI and MTI-2 could be members of a new class of plant serine proteinase inhibitors.
AB - A new serine proteinase inhibitor, rapeseed trypsin inhibitor (RTI), has been isolated from rapeseed (Brassica napus var. oleifera) seed. The protein inhibits the catalytic activity of bovine β-trypsin and bovine α-chymotrypsin with apparent dissociation constants of 3.0 × 10-10 M and 4.1 × 10-7 M, at pH 8.0 and 21°C, respectively. The stoichiometry of both proteinase-inhibitor complexes is 1:1. The amino acid sequence of RTI consists of 60 amino acid residues, corresponding to an Mr, of about 6.7 kDa. The p1-pi, reactive site bond has been tentatively identified at position Arg20-Ile21. RTI shows no similarity to other serine proteinase inhibitors except the low molecular weight mustard trypsin inhibitor (MTI-2). RTI and MTI-2 could be members of a new class of plant serine proteinase inhibitors.
KW - Amino acid sequence
KW - Brassica napus var. oleifera
KW - Rapeseed
KW - Serine proteinase inhibitor
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U2 - 10.1016/0014-5793(94)80505-9
DO - 10.1016/0014-5793(94)80505-9
M3 - Article
C2 - 8143882
AN - SCOPUS:0028219989
VL - 342
SP - 221
EP - 224
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -