Purification of α-1-antitrypsin monomer by preparative electrophoresis

F. Spada, G. Candiano, C. Sergi, G. M. Ghiggeri, F. Callea, R. Gusmano

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Abstract

Alfa-l-antitrypsin (α1AT) was purified by pseudoligand chromatography and preparative electrophoresis from the serum of a patient with α1AT AT deficiency. The combination of the two techniques yielded a high grade batch of α1AT monomer and this was successfully used to purify the protein from the serum of PiMIMl, PiMIM2, and PiZZ phenotype subjects. This procedure should facilitate structural studies of α1AT variants susceptible to intracellular accumulation.

Original languageEnglish
Pages (from-to)661-663
Number of pages3
JournalJournal of Clinical Pathology
Volume47
Issue number7
DOIs
Publication statusPublished - 1994

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ASJC Scopus subject areas

  • Health Policy
  • Pathology and Forensic Medicine

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