TY - JOUR
T1 - Purification of α-1-antitrypsin monomer by preparative electrophoresis
AU - Spada, F.
AU - Candiano, G.
AU - Sergi, C.
AU - Ghiggeri, G. M.
AU - Callea, F.
AU - Gusmano, R.
PY - 1994
Y1 - 1994
N2 - Alfa-l-antitrypsin (α1AT) was purified by pseudoligand chromatography and preparative electrophoresis from the serum of a patient with α1AT AT deficiency. The combination of the two techniques yielded a high grade batch of α1AT monomer and this was successfully used to purify the protein from the serum of PiMIMl, PiMIM2, and PiZZ phenotype subjects. This procedure should facilitate structural studies of α1AT variants susceptible to intracellular accumulation.
AB - Alfa-l-antitrypsin (α1AT) was purified by pseudoligand chromatography and preparative electrophoresis from the serum of a patient with α1AT AT deficiency. The combination of the two techniques yielded a high grade batch of α1AT monomer and this was successfully used to purify the protein from the serum of PiMIMl, PiMIM2, and PiZZ phenotype subjects. This procedure should facilitate structural studies of α1AT variants susceptible to intracellular accumulation.
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U2 - 10.1136/jcp.47.7.661
DO - 10.1136/jcp.47.7.661
M3 - Article
C2 - 8089226
AN - SCOPUS:0028631899
VL - 47
SP - 661
EP - 663
JO - Journal of Clinical Pathology - Clinical Molecular Pathology
JF - Journal of Clinical Pathology - Clinical Molecular Pathology
SN - 0021-9746
IS - 7
ER -