Purification of α-1-antitrypsin monomer by preparative electrophoresis

F. Spada; G. Candiano; C. Sergi; G. M. Ghiggeri; F. Callea; R. Gusmano

doi:10.1136/jcp.47.7.661

Purification of α-1-antitrypsin monomer by preparative electrophoresis

F. Spada, G. Candiano, C. Sergi, G. M. Ghiggeri, F. Callea, R. Gusmano

Research output: Contribution to journal › Article

Abstract

Alfa-l-antitrypsin (α₁AT) was purified by pseudoligand chromatography and preparative electrophoresis from the serum of a patient with α₁AT AT deficiency. The combination of the two techniques yielded a high grade batch of α₁AT monomer and this was successfully used to purify the protein from the serum of PiMIMl, PiMIM2, and PiZZ phenotype subjects. This procedure should facilitate structural studies of α₁AT variants susceptible to intracellular accumulation.

Original language	English
Pages (from-to)	661-663
Number of pages	3
Journal	Journal of Clinical Pathology
Volume	47
Issue number	7
DOIs	https://doi.org/10.1136/jcp.47.7.661
Publication status	Published - 1994

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ASJC Scopus subject areas

Health Policy
Pathology and Forensic Medicine

Cite this

Spada, F., Candiano, G., Sergi, C., Ghiggeri, G. M., Callea, F., & Gusmano, R. (1994). Purification of α-1-antitrypsin monomer by preparative electrophoresis. Journal of Clinical Pathology, 47(7), 661-663. https://doi.org/10.1136/jcp.47.7.661

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10.1136/jcp.47.7.661