Purification of 6-phosphogluconate dehydrogenase from human erythrocytes by blue dextran chromatography

R. Ravazzolo, G. Bruzzone, C. Garre

Research output: Contribution to journalArticle

Abstract

A simplified purification procedure of human red cell 6-PGD, based on Blue Dextran-Sepharose affinity chromatography, is described. The enzyme obtained with this procedure is homogeneous, and the specific activity is 9.0 U/mg protein after a 3,000 fold purification. The method should be particularly suitable for purification from small samples of blood as in the case of genetic variants.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
JournalBulletin of Molecular Biology and Medicine
Volume3
Issue number3
Publication statusPublished - 1978

ASJC Scopus subject areas

  • Clinical Biochemistry

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