Purification of immunomodulatory factors in human peripheral blood leukocytes

P. C. Montecucchi, R. Caldarini, M. Sansó, A. M. Bertolaso, V. E M Rosso Di San Secondo, G. Piccolo, G. Sirchia

Research output: Contribution to journalArticlepeer-review

Abstract

Procedure is described for purifying low-molecular-weight factors with antigenaspecific properties from a dialysate of human leukocyte extract. It includes gel chromatography on Sephade G-25 and G-15, ion-exchange chromatography, reversed-phase high-performance liquid chromatography (HPLC) on a C18 hydrophobic column and gel permeation HPLC . The immunosuppressive factor (mol.wt. 800-1000) was purified to near homogeneity. It is probably of peptide nature, although it is pronase resistant. The enhancer factor (mol.wt. 300-600) is eluted from chromatographic columns together with a hypoxanthine-like substance. Nevertheless, the biological activity cannot be attributed to the purine derivative. Identification of this amplifier activity is still lacking.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalJournal of Chromatography A
Volume512
Issue numberC
DOIs
Publication statusPublished - Jul 20 1990

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

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