Purification of immunomodulatory factors in human peripheral blood leukocytes

P. C. Montecucchi, R. Caldarini, M. Sansó, A. M. Bertolaso, V. E M Rosso Di San Secondo, G. Piccolo, G. Sirchia

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Procedure is described for purifying low-molecular-weight factors with antigenaspecific properties from a dialysate of human leukocyte extract. It includes gel chromatography on Sephade G-25 and G-15, ion-exchange chromatography, reversed-phase high-performance liquid chromatography (HPLC) on a C18 hydrophobic column and gel permeation HPLC . The immunosuppressive factor (mol.wt. 800-1000) was purified to near homogeneity. It is probably of peptide nature, although it is pronase resistant. The enhancer factor (mol.wt. 300-600) is eluted from chromatographic columns together with a hypoxanthine-like substance. Nevertheless, the biological activity cannot be attributed to the purine derivative. Identification of this amplifier activity is still lacking.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalJournal of Chromatography A
Volume512
Issue numberC
DOIs
Publication statusPublished - Jul 20 1990

Fingerprint

High performance liquid chromatography
Chromatography
Purification
Leukocytes
Blood
Gels
High Pressure Liquid Chromatography
Pronase
Hypoxanthine
Dialysis Solutions
Ion Exchange Chromatography
Reverse-Phase Chromatography
Immunosuppressive Agents
Bioactivity
Permeation
Gel Chromatography
Ion exchange
Molecular Weight
Molecular weight
Derivatives

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Montecucchi, P. C., Caldarini, R., Sansó, M., Bertolaso, A. M., Di San Secondo, V. E. M. R., Piccolo, G., & Sirchia, G. (1990). Purification of immunomodulatory factors in human peripheral blood leukocytes. Journal of Chromatography A, 512(C), 139-147. https://doi.org/10.1016/S0021-9673(01)89480-2

Purification of immunomodulatory factors in human peripheral blood leukocytes. / Montecucchi, P. C.; Caldarini, R.; Sansó, M.; Bertolaso, A. M.; Di San Secondo, V. E M Rosso; Piccolo, G.; Sirchia, G.

In: Journal of Chromatography A, Vol. 512, No. C, 20.07.1990, p. 139-147.

Research output: Contribution to journalArticle

Montecucchi, PC, Caldarini, R, Sansó, M, Bertolaso, AM, Di San Secondo, VEMR, Piccolo, G & Sirchia, G 1990, 'Purification of immunomodulatory factors in human peripheral blood leukocytes', Journal of Chromatography A, vol. 512, no. C, pp. 139-147. https://doi.org/10.1016/S0021-9673(01)89480-2
Montecucchi PC, Caldarini R, Sansó M, Bertolaso AM, Di San Secondo VEMR, Piccolo G et al. Purification of immunomodulatory factors in human peripheral blood leukocytes. Journal of Chromatography A. 1990 Jul 20;512(C):139-147. https://doi.org/10.1016/S0021-9673(01)89480-2
Montecucchi, P. C. ; Caldarini, R. ; Sansó, M. ; Bertolaso, A. M. ; Di San Secondo, V. E M Rosso ; Piccolo, G. ; Sirchia, G. / Purification of immunomodulatory factors in human peripheral blood leukocytes. In: Journal of Chromatography A. 1990 ; Vol. 512, No. C. pp. 139-147.
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