Purification of interleukin-2 antibodies from healthy individuals

Eugenio Monti, Ambra Pozzi, Laura Tiberio, Daniele Morelli, Arnaldo Caruso, Maria Luisa Villa, Andrea Balsari

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

By covalent binding of recombinant interleukin-2 (rIL-2) to Sepharose, it was possible to immunopurify specific human anti-IL-2 antibodies from a pool of immunoglobulins obtained from healthy subjects. Since low quantities of the ligand released by the matrix could interfere with the evaluation of the biological activity of anti-IL-2 antibodies, the antibody preparation was subjected to pepsin digestion which is known to destroy the IL-2 molecule. Purified human anti-IL-2 antibodies were found to be mostly IgG1 and able to neutralize IL-2 induced peripheral blood lymphocytes (PBL) proliferation in vitro. The availability of purified anti-IL-2 antibodies, obtained from healthy individuals, able to modulate IL-2 activity, could be important in several therapeutic approaches.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalImmunology Letters
Volume36
Issue number3
DOIs
Publication statusPublished - 1993

Fingerprint

Interleukin-2
Antibodies
Pepsin A
Sepharose
Immunoglobulins
Digestion
Healthy Volunteers
Immunoglobulin G
Lymphocytes
Ligands

Keywords

  • Affinity chromatography
  • Interleukin 2
  • Natural antibody

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Monti, E., Pozzi, A., Tiberio, L., Morelli, D., Caruso, A., Villa, M. L., & Balsari, A. (1993). Purification of interleukin-2 antibodies from healthy individuals. Immunology Letters, 36(3), 261-266. https://doi.org/10.1016/0165-2478(93)90098-M

Purification of interleukin-2 antibodies from healthy individuals. / Monti, Eugenio; Pozzi, Ambra; Tiberio, Laura; Morelli, Daniele; Caruso, Arnaldo; Villa, Maria Luisa; Balsari, Andrea.

In: Immunology Letters, Vol. 36, No. 3, 1993, p. 261-266.

Research output: Contribution to journalArticle

Monti, E, Pozzi, A, Tiberio, L, Morelli, D, Caruso, A, Villa, ML & Balsari, A 1993, 'Purification of interleukin-2 antibodies from healthy individuals', Immunology Letters, vol. 36, no. 3, pp. 261-266. https://doi.org/10.1016/0165-2478(93)90098-M
Monti, Eugenio ; Pozzi, Ambra ; Tiberio, Laura ; Morelli, Daniele ; Caruso, Arnaldo ; Villa, Maria Luisa ; Balsari, Andrea. / Purification of interleukin-2 antibodies from healthy individuals. In: Immunology Letters. 1993 ; Vol. 36, No. 3. pp. 261-266.
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