Purification of tenascin from conditioned tissue culture media

Annalisa Siri, Enrica Balza, Barbara Gaggero, Luciano Zardi

Research output: Contribution to journalArticle

Abstract

We describe a purification procedure of human tenascin from conditioned medium of SK-MEL-28 melanoma cell line which involves the use of a hydroxyapatite chromatography column followed by polyethyleneglycol precipitation of crude tenascin eluted from the hydroxyapatite column. This procedure does not involve the use of specific antibodies and can thus be applied to culture media of cells of different species.

Original languageEnglish
Pages (from-to)201-204
Number of pages4
JournalJournal of Tissue Culture Methods
Volume16
Issue number3-4
DOIs
Publication statusPublished - Sep 1994

Fingerprint

Tenascin
Durapatite
Conditioned Culture Medium
Culture Media
Chromatography
Melanoma
Cell Line
Antibodies

Keywords

  • Fibronectin
  • Hydroxyapatite
  • Polyethyleneglycol
  • Tenascin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Purification of tenascin from conditioned tissue culture media. / Siri, Annalisa; Balza, Enrica; Gaggero, Barbara; Zardi, Luciano.

In: Journal of Tissue Culture Methods, Vol. 16, No. 3-4, 09.1994, p. 201-204.

Research output: Contribution to journalArticle

Siri, Annalisa ; Balza, Enrica ; Gaggero, Barbara ; Zardi, Luciano. / Purification of tenascin from conditioned tissue culture media. In: Journal of Tissue Culture Methods. 1994 ; Vol. 16, No. 3-4. pp. 201-204.
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