Purification to homogeneity and biochemical characterization of two suppressor factors from human malignant T-cells

M. Ponzoni, P. G. Montaldo, M. Lanciotti, E. Castagnola, C. Cirillo, P. Cornaglia-Ferraris

Research output: Contribution to journalArticlepeer-review

Abstract

A cell clone (GI-CO-T-9) derived from a long term T-cell culture (PF-382), established from a patient affected by acute T-lymphoblastic leukemia (T-ALL), was selected for the presence in the culture medium of factors suppressing T-cell proliferation. The crude supernatant has been subjected to a multi-step chromatographic fractioning, including: preparative gel permeation, anion exchange, and hydrophobic interaction High Performance Liquid Chromatography (HPLC). The highly purified material was characterized by polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE), revealing single bands of 115 Kd and 80 Kd. The isoelectric points (pI), determined by flat-bed isoelectricfocusing, were 7.4 for High Molecular Weight Suppressor Factor (HMWSF) and 3.5-3.6 for Low Molecular Weight Suppressor Factor (LMWSF).

Original languageEnglish
Pages (from-to)702-710
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume150
Issue number2
DOIs
Publication statusPublished - Jan 29 1988

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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