Purification to homogeneity of alpha (III) collagen chain from human placenta by preparative electrophoresis.

G. Candiano, P. Altieri, R. Bertelli, A. Garberi, G. M. Ghiggeri

Research output: Contribution to journalArticle

Abstract

A multiple electrophoretic method suitable for purification of type III collagen from a mixture of various collagen types is described. The technique is based on preparative electrophoresis in acid media and successive electroelution of the collagen fractions. By sodium dodecyl sulfate-polyacrylamide electrophoresis and immunoblotting, the collagen composition of the electroeluted fractions was characterized as a mixture of alpha (III), beta (III) and gamma (III) without any other visible contaminants. This technique enabled us to obtain large quantities of type III collagen from partial pepsin digests of human placenta suitable for further processing.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalApplied and theoretical electrophoresis : the official journal of the International Electrophoresis Society
Volume2
Issue number2-3
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Medicine(all)

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