Abstract
Problem: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM- and VT-/PM+ subjects on human first-trimester trophoblast (HTR8) cells. Method of study: HTR-8 cells were incubated with APS VT+/PM-, APS VT-/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. Results: VT-/PM+ IgG, but not VT+/PM- IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM- nor VT-/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. Conclusions: VT-/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM- IgG do not.
Original language | English |
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Pages (from-to) | 390-401 |
Number of pages | 12 |
Journal | American Journal of Reproductive Immunology |
Volume | 73 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 1 2015 |
Keywords
- Antiphospholipid
- Obstetric
- TLR4
- Trophoblast
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Obstetrics and Gynaecology
- Reproductive Medicine