Purified IgG from patients with obstetric but not IgG from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion

Katie Poulton; Vera M. Ripoll; Charis Pericleous; Pier Luigi Meroni; Maria Gerosa; Yiannis Ioannou; Anisur Rahman; Ian P. Giles

doi:10.1111/aji.12341

Purified IgG from patients with obstetric but not IgG from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion

Katie Poulton, Vera M. Ripoll, Charis Pericleous, Pier Luigi Meroni, Maria Gerosa, Yiannis Ioannou, Anisur Rahman, Ian P. Giles

Fondazione Istituto Auxologico Italiano

Research output: Contribution to journal › Article › peer-review

Abstract

Problem: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM- and VT-/PM+ subjects on human first-trimester trophoblast (HTR8) cells. Method of study: HTR-8 cells were incubated with APS VT+/PM-, APS VT-/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. Results: VT-/PM+ IgG, but not VT+/PM- IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM- nor VT-/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. Conclusions: VT-/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM- IgG do not.

Original language	English
Pages (from-to)	390-401
Number of pages	12
Journal	American Journal of Reproductive Immunology
Volume	73
Issue number	5
DOIs	https://doi.org/10.1111/aji.12341
Publication status	Published - May 1 2015

Keywords

Antiphospholipid
Obstetric
TLR4
Trophoblast

ASJC Scopus subject areas

Immunology
Immunology and Allergy
Obstetrics and Gynaecology
Reproductive Medicine

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10.1111/aji.12341

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Purified IgG from patients with obstetric but not IgG from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion. / Poulton, Katie; Ripoll, Vera M.; Pericleous, Charis; Meroni, Pier Luigi; Gerosa, Maria; Ioannou, Yiannis; Rahman, Anisur; Giles, Ian P.

In: American Journal of Reproductive Immunology, Vol. 73, No. 5, 01.05.2015, p. 390-401.

Research output: Contribution to journal › Article › peer-review

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abstract = "Problem: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM- and VT-/PM+ subjects on human first-trimester trophoblast (HTR8) cells. Method of study: HTR-8 cells were incubated with APS VT+/PM-, APS VT-/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. Results: VT-/PM+ IgG, but not VT+/PM- IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM- nor VT-/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. Conclusions: VT-/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM- IgG do not.",

keywords = "Antiphospholipid, Obstetric, TLR4, Trophoblast",

author = "Katie Poulton and Ripoll, {Vera M.} and Charis Pericleous and Meroni, {Pier Luigi} and Maria Gerosa and Yiannis Ioannou and Anisur Rahman and Giles, {Ian P.}",

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AU - Poulton, Katie

AU - Ripoll, Vera M.

AU - Pericleous, Charis

AU - Meroni, Pier Luigi

AU - Gerosa, Maria

AU - Ioannou, Yiannis

AU - Rahman, Anisur

AU - Giles, Ian P.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Problem: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM- and VT-/PM+ subjects on human first-trimester trophoblast (HTR8) cells. Method of study: HTR-8 cells were incubated with APS VT+/PM-, APS VT-/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. Results: VT-/PM+ IgG, but not VT+/PM- IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM- nor VT-/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. Conclusions: VT-/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM- IgG do not.

AB - Problem: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM- and VT-/PM+ subjects on human first-trimester trophoblast (HTR8) cells. Method of study: HTR-8 cells were incubated with APS VT+/PM-, APS VT-/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. Results: VT-/PM+ IgG, but not VT+/PM- IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM- nor VT-/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. Conclusions: VT-/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM- IgG do not.

KW - Antiphospholipid

KW - Obstetric

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KW - Trophoblast

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Purified IgG from patients with obstetric but not IgG from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion

Abstract

Keywords

ASJC Scopus subject areas

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