Purine receptors in the guinea-pig internal anal sphincter

A. Crema, G. M. Frigo, S. Lecchini, L. Manzo, L. Onori, M. Tonini

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In the isolated internal anal sphincter of the guinea-pig, adenosine 5'-triphosphate (ATP) and adenosine induced a concentration-dependent and tetrodotoxin-insensitive relaxation. Pretreatment with theophylline (25-50 μM) had no significant effect on the concentration-response curves obtained with either purine compound. Reactive blue 2 (25-100 μM) shifted the curve to ATP to the right in a dose-dependent fashion leaving that to adenosine unaltered. The antagonism appeared to be non-competitive. Neither reactive blue 2 nor purine receptor occupation by ATP or adenosine altered the electrically-induced non-adrenergic, non-cholinergic inhibitory response. The actions of ATP and adenosine in the guinea-pig internal anal sphincter appear to be mediated by separate receptors. These receptors are not involved in the nerve-mediated relaxation.

Original languageEnglish
Pages (from-to)599-603
Number of pages5
JournalBritish Journal of Pharmacology
Issue number3
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Pharmacology

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    Crema, A., Frigo, G. M., Lecchini, S., Manzo, L., Onori, L., & Tonini, M. (1983). Purine receptors in the guinea-pig internal anal sphincter. British Journal of Pharmacology, 78(3), 599-603.