Pyothorax-associated lymphoma: Description of the first two cases detected in Italy

S. Ascani, M. Piccioli, S. Poggi, A. Briskomatis, G. B. Bolis, F. Liberati, R. Frongillo, C. Caramatti, G. Fraternali-Orcioni, B. Gamberi, P. L. Zinzani, S. Lazzi, L. Leoncini, J. O'Leary, P. P. Piccaluga, S. A. Pileri

Research output: Contribution to journalArticle

Abstract

Background: Pyothorax-associated lymphoma (PAL) is a rare, but distinct, clinico-pathologic entity which occurs most often in Japanese people; to the best of our knowledge, only six cases of it have been reported in Western countries. The tumour develops several decades following artificial pneumothorax or chronic pleuritis due to tuberculous infection, produces pleural effusion associated with extensive local lymphomatous infiltrates, and is sustained by a polymorphic large B-cell clonal proliferation showing EBV integration in the genome of the neoplastic cells. Patients and methods: Herein we describe two cases of PAL observed in Italian patients, both extensively studied on the clinical, pathological, phenotypic, virological, and molecular levels. Results: The two cases occurred, respectively, 45 and 50 years after therapeutic pneumothorax because of tuberculous pleuritis and were characterized by a pleural mass extending to the thoracic wall, which on histological examination were seen to consist of large elements with immunoblastic morphology. Immunohistochemistry showed monotypic restriction of Ig light chains, as well as the expression of CD45, B-cell markers (CD20, CD79a, CD45RA), bcl-2 oncogene product, EBNA-2 and, partially, LMP-1. The ratio of cycling cells was extremely high as was the number of mitotic figures. In situ hybridization displayed the presence in the neoplastic cells of the EBV-related small RNAs EBER 1 and 2, which in turn, along with the positivity for EBNA-2 and LMP-1, further strengthened the close relationships between PAL and latent viral infection. Molecular studies revealed, on one hand, clonal rearrangement of the Ig heavy chain J region genes, and on the other, negativity for HHV8 in one case and positivity in the other. Conclusions: These cases of PAL are the first to be documented in Italy; they serve to direct attention to the fact that this condition is not confined to Japanese people, and that its occurrence in Western countries might be underestimated.

Original languageEnglish
Pages (from-to)1133-1138
Number of pages6
JournalAnnals of Oncology
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 1997

Fingerprint

Pleural Empyema
Italy
Lymphoma
Pleurisy
Human Herpesvirus 4
B-Lymphocytes
Artificial Pneumothorax
Immunoglobulin Heavy Chains
Oncogene Proteins
Thoracic Wall
Pneumothorax
Virus Diseases
Pleural Effusion
In Situ Hybridization
Immunohistochemistry
Cell Proliferation
Genome
RNA
Light
Infection

Keywords

  • EBV
  • Immunohistochemistry
  • Malignant lymphoma
  • Molecular biology
  • Pleuritis
  • Pneumothorax
  • Pyothorax
  • Tuberculosis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Ascani, S., Piccioli, M., Poggi, S., Briskomatis, A., Bolis, G. B., Liberati, F., ... Pileri, S. A. (1997). Pyothorax-associated lymphoma: Description of the first two cases detected in Italy. Annals of Oncology, 8(11), 1133-1138. https://doi.org/10.1023/A:1008285708096

Pyothorax-associated lymphoma : Description of the first two cases detected in Italy. / Ascani, S.; Piccioli, M.; Poggi, S.; Briskomatis, A.; Bolis, G. B.; Liberati, F.; Frongillo, R.; Caramatti, C.; Fraternali-Orcioni, G.; Gamberi, B.; Zinzani, P. L.; Lazzi, S.; Leoncini, L.; O'Leary, J.; Piccaluga, P. P.; Pileri, S. A.

In: Annals of Oncology, Vol. 8, No. 11, 11.1997, p. 1133-1138.

Research output: Contribution to journalArticle

Ascani, S, Piccioli, M, Poggi, S, Briskomatis, A, Bolis, GB, Liberati, F, Frongillo, R, Caramatti, C, Fraternali-Orcioni, G, Gamberi, B, Zinzani, PL, Lazzi, S, Leoncini, L, O'Leary, J, Piccaluga, PP & Pileri, SA 1997, 'Pyothorax-associated lymphoma: Description of the first two cases detected in Italy', Annals of Oncology, vol. 8, no. 11, pp. 1133-1138. https://doi.org/10.1023/A:1008285708096
Ascani S, Piccioli M, Poggi S, Briskomatis A, Bolis GB, Liberati F et al. Pyothorax-associated lymphoma: Description of the first two cases detected in Italy. Annals of Oncology. 1997 Nov;8(11):1133-1138. https://doi.org/10.1023/A:1008285708096
Ascani, S. ; Piccioli, M. ; Poggi, S. ; Briskomatis, A. ; Bolis, G. B. ; Liberati, F. ; Frongillo, R. ; Caramatti, C. ; Fraternali-Orcioni, G. ; Gamberi, B. ; Zinzani, P. L. ; Lazzi, S. ; Leoncini, L. ; O'Leary, J. ; Piccaluga, P. P. ; Pileri, S. A. / Pyothorax-associated lymphoma : Description of the first two cases detected in Italy. In: Annals of Oncology. 1997 ; Vol. 8, No. 11. pp. 1133-1138.
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AU - Ascani, S.

AU - Piccioli, M.

AU - Poggi, S.

AU - Briskomatis, A.

AU - Bolis, G. B.

AU - Liberati, F.

AU - Frongillo, R.

AU - Caramatti, C.

AU - Fraternali-Orcioni, G.

AU - Gamberi, B.

AU - Zinzani, P. L.

AU - Lazzi, S.

AU - Leoncini, L.

AU - O'Leary, J.

AU - Piccaluga, P. P.

AU - Pileri, S. A.

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