Pyropheophorbide-a methyl ester-mediated photosensitization activates transcription factor NF-κB through the interleukin-1 receptor-dependent signaling pathway

Jean Yves Matroule, Giuzeppina Bonizzi, Patrice Morlière, Nicole Paillous, René Santus, Vincent Bours, Jacques Piette

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Pyropheophorbide-a methyl ester (PPME) is a second generation of photosensitizers used in photodynamic therapy. We demonstrated that PPME photosensitization activated NF-κB transcription factor in colon cancer cells. Unexpectedly, this activation occurred in two separate waves, i.e. a rapid and transient one and a second slower but sustained phase. The former was due to photosensitization by PPME localized in the cytoplasmic membrane which triggered interleukin-1 receptor internalization and the transduction pathways controlled by the interleukin-1 type I receptor. Indeed, TRAF6 dominant negative mutant abolished NF-κB activation by PPME photosensitization, and TRAF2 dominant negative mutant was without any effect, and overexpression of IκB kinases increased gene transcription controlled by NF-κB. Oxidative stress was not likely involved in the activation. On the other hand, the slower and sustained wave could be the product of the release of ceramide through activation of the acidic sphingomyelinase. PPME localization within the lysosomal membrane could explain why ceramide acted as second messenger in NF-κB activation by PPME photosensitization. These data will allow a better understanding of the molecular basis of tumor eradication by photodynamic therapy, in particular the importance of the host cell response in the treatment.

Original languageEnglish
Pages (from-to)2988-3000
Number of pages13
JournalJournal of Biological Chemistry
Volume274
Issue number5
DOIs
Publication statusPublished - Jan 29 1999

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Photosensitivity Disorders
Interleukin-1 Receptors
Esters
Transcription Factors
Chemical activation
Photodynamic therapy
Ceramides
Photochemotherapy
Interleukin-1 Type I Receptors
TNF Receptor-Associated Factor 2
TNF Receptor-Associated Factor 6
Membranes
Sphingomyelin Phosphodiesterase
Oxidative stress
Photosensitizing Agents
Second Messenger Systems
Transcription
Colonic Neoplasms
pyropheophorbide a
Tumors

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pyropheophorbide-a methyl ester-mediated photosensitization activates transcription factor NF-κB through the interleukin-1 receptor-dependent signaling pathway. / Matroule, Jean Yves; Bonizzi, Giuzeppina; Morlière, Patrice; Paillous, Nicole; Santus, René; Bours, Vincent; Piette, Jacques.

In: Journal of Biological Chemistry, Vol. 274, No. 5, 29.01.1999, p. 2988-3000.

Research output: Contribution to journalArticle

Matroule, Jean Yves ; Bonizzi, Giuzeppina ; Morlière, Patrice ; Paillous, Nicole ; Santus, René ; Bours, Vincent ; Piette, Jacques. / Pyropheophorbide-a methyl ester-mediated photosensitization activates transcription factor NF-κB through the interleukin-1 receptor-dependent signaling pathway. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 5. pp. 2988-3000.
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