Pyrrolidine dithiocarbamate reduced experimental periodontitis

Carmelo Muià, Emanuela Mazzon, Daniele Maiere, Daniele Zito, Rosanna Di Paola, Santori Domenico, Concetta Crisafulli, Domenico Britti, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Nuclear factor-κB (NF-κB) is a transcription factor which plays a pivotal role in the induction of genes involved in physiological processes as well as in the response to injury and inflammation. Dithiocarbamates are antioxidants which are potent inhibitors of NF-κB. We postulated that pyrrolidine dithiocarbamate (PDTC) would attenuate inflammation. In the present study, we have investigated the effects of PDTC, in a rat model of periodontitis. Periodontitis was induced in rats by placing around the lower left first molar a 2/0 braided silk. At day eight the gingivomucosal tissue encircling the mandibular first molar was removed for biochemical and histological analysis. At day eight ligations significantly induced an increase neutrophil infiltration as well as the gingivomucosal tissue expression of TNF-α and iNOS as well as nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Intraperitonial injection of PDTC (10 mg/kg daily for eight days) significantly reduced all of the parameters of inflammation as described above. These data demonstrate that PDTC exerts an anti-inflammatory role during experimental periodontitis and is able to ameliorate the tissue damage associated with ligature-induced periodontitis.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalEuropean Journal of Pharmacology
Volume539
Issue number3
DOIs
Publication statusPublished - Jun 13 2006

Fingerprint

Periodontitis
Ligation
Inflammation
Physiological Phenomena
Evans Blue
Silk
Poly(ADP-ribose) Polymerases
Neutrophil Infiltration
Anti-Inflammatory Agents
Transcription Factors
Antioxidants
Bone and Bones
Injections
pyrrolidine dithiocarbamic acid
Wounds and Injuries
Genes

Keywords

  • Alveolar bone loss
  • Nuclear factor-κB
  • Periodontal disease
  • Pyrrolidine dithiocarbamate
  • Reactive oxygen specie

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Muià, C., Mazzon, E., Maiere, D., Zito, D., Di Paola, R., Domenico, S., ... Cuzzocrea, S. (2006). Pyrrolidine dithiocarbamate reduced experimental periodontitis. European Journal of Pharmacology, 539(3), 205-210. https://doi.org/10.1016/j.ejphar.2006.03.072

Pyrrolidine dithiocarbamate reduced experimental periodontitis. / Muià, Carmelo; Mazzon, Emanuela; Maiere, Daniele; Zito, Daniele; Di Paola, Rosanna; Domenico, Santori; Crisafulli, Concetta; Britti, Domenico; Cuzzocrea, Salvatore.

In: European Journal of Pharmacology, Vol. 539, No. 3, 13.06.2006, p. 205-210.

Research output: Contribution to journalArticle

Muià, C, Mazzon, E, Maiere, D, Zito, D, Di Paola, R, Domenico, S, Crisafulli, C, Britti, D & Cuzzocrea, S 2006, 'Pyrrolidine dithiocarbamate reduced experimental periodontitis', European Journal of Pharmacology, vol. 539, no. 3, pp. 205-210. https://doi.org/10.1016/j.ejphar.2006.03.072
Muià, Carmelo ; Mazzon, Emanuela ; Maiere, Daniele ; Zito, Daniele ; Di Paola, Rosanna ; Domenico, Santori ; Crisafulli, Concetta ; Britti, Domenico ; Cuzzocrea, Salvatore. / Pyrrolidine dithiocarbamate reduced experimental periodontitis. In: European Journal of Pharmacology. 2006 ; Vol. 539, No. 3. pp. 205-210.
@article{d4405f6dd1e34c068da3de91da1fc612,
title = "Pyrrolidine dithiocarbamate reduced experimental periodontitis",
abstract = "Nuclear factor-κB (NF-κB) is a transcription factor which plays a pivotal role in the induction of genes involved in physiological processes as well as in the response to injury and inflammation. Dithiocarbamates are antioxidants which are potent inhibitors of NF-κB. We postulated that pyrrolidine dithiocarbamate (PDTC) would attenuate inflammation. In the present study, we have investigated the effects of PDTC, in a rat model of periodontitis. Periodontitis was induced in rats by placing around the lower left first molar a 2/0 braided silk. At day eight the gingivomucosal tissue encircling the mandibular first molar was removed for biochemical and histological analysis. At day eight ligations significantly induced an increase neutrophil infiltration as well as the gingivomucosal tissue expression of TNF-α and iNOS as well as nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Intraperitonial injection of PDTC (10 mg/kg daily for eight days) significantly reduced all of the parameters of inflammation as described above. These data demonstrate that PDTC exerts an anti-inflammatory role during experimental periodontitis and is able to ameliorate the tissue damage associated with ligature-induced periodontitis.",
keywords = "Alveolar bone loss, Nuclear factor-κB, Periodontal disease, Pyrrolidine dithiocarbamate, Reactive oxygen specie",
author = "Carmelo Mui{\`a} and Emanuela Mazzon and Daniele Maiere and Daniele Zito and {Di Paola}, Rosanna and Santori Domenico and Concetta Crisafulli and Domenico Britti and Salvatore Cuzzocrea",
year = "2006",
month = "6",
day = "13",
doi = "10.1016/j.ejphar.2006.03.072",
language = "English",
volume = "539",
pages = "205--210",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Pyrrolidine dithiocarbamate reduced experimental periodontitis

AU - Muià, Carmelo

AU - Mazzon, Emanuela

AU - Maiere, Daniele

AU - Zito, Daniele

AU - Di Paola, Rosanna

AU - Domenico, Santori

AU - Crisafulli, Concetta

AU - Britti, Domenico

AU - Cuzzocrea, Salvatore

PY - 2006/6/13

Y1 - 2006/6/13

N2 - Nuclear factor-κB (NF-κB) is a transcription factor which plays a pivotal role in the induction of genes involved in physiological processes as well as in the response to injury and inflammation. Dithiocarbamates are antioxidants which are potent inhibitors of NF-κB. We postulated that pyrrolidine dithiocarbamate (PDTC) would attenuate inflammation. In the present study, we have investigated the effects of PDTC, in a rat model of periodontitis. Periodontitis was induced in rats by placing around the lower left first molar a 2/0 braided silk. At day eight the gingivomucosal tissue encircling the mandibular first molar was removed for biochemical and histological analysis. At day eight ligations significantly induced an increase neutrophil infiltration as well as the gingivomucosal tissue expression of TNF-α and iNOS as well as nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Intraperitonial injection of PDTC (10 mg/kg daily for eight days) significantly reduced all of the parameters of inflammation as described above. These data demonstrate that PDTC exerts an anti-inflammatory role during experimental periodontitis and is able to ameliorate the tissue damage associated with ligature-induced periodontitis.

AB - Nuclear factor-κB (NF-κB) is a transcription factor which plays a pivotal role in the induction of genes involved in physiological processes as well as in the response to injury and inflammation. Dithiocarbamates are antioxidants which are potent inhibitors of NF-κB. We postulated that pyrrolidine dithiocarbamate (PDTC) would attenuate inflammation. In the present study, we have investigated the effects of PDTC, in a rat model of periodontitis. Periodontitis was induced in rats by placing around the lower left first molar a 2/0 braided silk. At day eight the gingivomucosal tissue encircling the mandibular first molar was removed for biochemical and histological analysis. At day eight ligations significantly induced an increase neutrophil infiltration as well as the gingivomucosal tissue expression of TNF-α and iNOS as well as nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Intraperitonial injection of PDTC (10 mg/kg daily for eight days) significantly reduced all of the parameters of inflammation as described above. These data demonstrate that PDTC exerts an anti-inflammatory role during experimental periodontitis and is able to ameliorate the tissue damage associated with ligature-induced periodontitis.

KW - Alveolar bone loss

KW - Nuclear factor-κB

KW - Periodontal disease

KW - Pyrrolidine dithiocarbamate

KW - Reactive oxygen specie

UR - http://www.scopus.com/inward/record.url?scp=33646859910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646859910&partnerID=8YFLogxK

U2 - 10.1016/j.ejphar.2006.03.072

DO - 10.1016/j.ejphar.2006.03.072

M3 - Article

VL - 539

SP - 205

EP - 210

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 3

ER -