qPCR Applications for the Determination of the Biological Age

Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello, Nicoletta Sacchi

Research output: Contribution to journalArticlepeer-review


Individual age is a phenotypic trait that provides useful information not only in forensic investigations but also in the aging research which is becoming an urgent call due to the dramatic growth of the aging population worldwide.TaqMan quantification PCR (qPCR) can be successfully applied to biological age estimation, using method defined in Zubakov et al. (Curr Biol 20:R970-R971, 2010). Since levels of signal joint T-cell receptor rearrangement excision circle (sjTREC) in human lymphocytes are known to decrease with age increasing, the qPCR of sjTREC represents a simple and relatively reproducible technique which offers highly accurate age estimation results.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - Jan 1 2020


  • Aging
  • Lymphocytes
  • qPCR
  • sjTREC

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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