Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34+ cells with small- and large-scale Miltenyi cell sorting system

Claudia Voena, Giovanni Locatelli, Claudia Castellino, Paola Omedè, Marco Ladetto, Elisabetta Zappone, Raffaella Milani, Vittorio Perfetti, Mario Boccadoro, Alessandro Pileri, Paolo Lusso, Chiara Villa, Mauro Malnati, Paolo Corradini

Research output: Contribution to journalArticlepeer-review

Abstract

The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.

Original languageEnglish
Pages (from-to)642-645
Number of pages4
JournalBritish Journal of Haematology
Volume117
Issue number3
DOIs
Publication statusPublished - 2002

Keywords

  • CD34 cell selection
  • Immunoglobulin genes
  • Multiple myeloma
  • Taqman PCR

ASJC Scopus subject areas

  • Hematology

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