Abstract
The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.
Original language | English |
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Pages (from-to) | 642-645 |
Number of pages | 4 |
Journal | British Journal of Haematology |
Volume | 117 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2002 |
Keywords
- CD34 cell selection
- Immunoglobulin genes
- Multiple myeloma
- Taqman PCR
ASJC Scopus subject areas
- Hematology