The yield and the post-transfusion recovery of random platelet concentrates (PC) are influenced by several variables. Platelet activation and damage of membrane associated receptorial complexes may occur during the preparation of platelet concentrate; during storage, serial changes in platelet ultrastructure, physicochemical and membrane properties occur; physico-chemical properties of the blood bags in which platelets are stored may variably affect either platelet preparation or storage. Therefore, the validation of any new plastic container for PC is based on data of quality control of the preparation and storage of platelet, which must explorate at least membrane glycoprotein (GP) expression and function, the appearance of platelet activation and lysis markers. The integrity of the GPIb-IX and GPIIb-IIIa complexes (which act as receptor for yon Willebrand factor, fibrinogen and other adhesive proteins), is required for the haemostatic efficiency of platelet. The membrane expression of P-selectin, an adhesion molecule, in the selectin family, which is normally located inside the αgranule membrane of the platelet, is considered a reliable index of platelet activation. The loss of membrane GPIb can be studied by monitoring the progressive increase in the supernatant of glycocalicin (GC), which is its 45 Kd aminoterminal portion. We studied the characteristics of platelet preparation (15 PC) and storage in a new bag for PC (manufactured by Grifols Laboratories, Murcia, Spain) which is specially intended to maintain oxygen content and pH and to minimize the release of plastic components. At the time of preparation and during an extended storage (up to 7 days), we have explored the above mentioned platelet properties, in a quality control program, which includes also control procedures routinarily performed at our Blood Bank for PC preparation and storage (lactate dehydrogenase, pH, platelet and leucocyte count). As control, we have evaluated 15 platelet concentrates separated and stored in Fenwal (PL1240) bags. The expression of GPIIb-IIIa seems to be unmodified, while a higher decrease of GPIb and a higher increase of GC value have been noticed in Fenwal as compared to Grifols bags. Moreover, a better pH maintenance (never lower than 6.8 during storage) and lower activation indexes (P-selectin, GC) characterize PC stored in Grifols bags. We conclude that the new oxygen-permeable Grifols bag shows platelet quality at least comparable to the conventional bags intended for prolonged platelet storage.
|Translated title of the contribution||Quality control in platelet concentrates: Validation of a new bag type|
|Number of pages||6|
|Journal||Trasfusione del Sangue|
|Publication status||Published - 1998|
ASJC Scopus subject areas
- Immunology and Allergy