Quantification and identification of polyomavirus DNA in blood and urine of renal transplant recipients

Fausto Baldanti, Gian Battista Fogazzi, Milena Furione, Lucia Saglimbeni, Francesca Rovida, Marta Gatti, Stefania Paolucci, Antonio Tarantino, Giuseppe Gerna

Research output: Contribution to journalArticle

Abstract

A cohort of 201 kidney transplant recipients (KTR) including 7 patients with evidence of renal function deterioration (as defined by creatinine levels >20% over baseline values) was analyzed for polyomavirus DNA in blood and urine samples by a new quantitative polymerase chain reaction method. Of 201 patients, 14 (6.9%) were positive for polyomavirus DNA in blood (median level, 500 copies per milliliter of blood) including all 7 patients with renal function deterioration. Polyomavirus DNA detection in blood for diagnosis of renal function deterioration in KTR showed a sensitivity of 100% and a specificity of 96%, whereas positive and negative predictive values were 50% and 100%, respectively. Diagnostic value of decoy cells detection and polyomavirus DNA quantification in urine samples was negligible.

Original languageEnglish
Pages (from-to)301-307
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume57
Issue number3
DOIs
Publication statusPublished - Mar 2007

Keywords

  • BK virus
  • DNA quantification
  • JC virus
  • Kidney transplant
  • PCR
  • Polyomavirus
  • Predictive value

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

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