Quantification of 4,4′-diaminodiphenylmethane by gas chromatography negative ion chemical lonization mass spectrometry

Emilio Benfenati, Marco Natangelo, Roberto Fanelli, Gabriele Lualdi, Rosa Tridico

Research output: Contribution to journalArticle

Abstract

4,4′-Diaminodiphenylmethane (DDM) is an aromatic amine commonly used as a chemical intermediate in the production of isocyanates, polyurethanes, polymers, and elastomers. This compound is a carcinogen in laboratory animals, but no definitive data are available regarding its carcinogenicity in humans. On account of its diffusion and toxicity, specific analytical methods are required for monitoring human exposure to this compound. Methods for its quantification in biological fluids using gas chromatography-mass spectrometry (GC-MS) have been developed, using electron impact (EI) and negative ion chemical ionization (NICI). Here we described a GC-NICI-MS analytical procedure for DDM in acqueous matrices. A tetradeuterated analogue of DDM (DDM-d4) was prepared in our laboratories with a simple proton-exchange reaction using deuterohydrochloric acid in deuterated methanol. This compound was used as internal standard. The samples were added with this internal standard, extracted with a solid phase column, and derivatized with a fluorinated anhydride. The final step was the GC-MS analysis of the resulting amide using the selected ion recording (SIR) mode. The use of NICI-MS as a GC detector gave high sensitivity, on account of the high response of the fluorinated species present in this type of ionization. Also, the EI ionization provided useful results and represents an alternative method. Furthermore, the accuracy of the method was guaranteed by the use of the deuterated internal Standard.

Original languageEnglish
Pages (from-to)352-359
Number of pages8
JournalMicrochemical Journal
Volume46
Issue number3
DOIs
Publication statusPublished - 1992

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Gas chromatography
Ionization
Mass spectrometry
Negative ions
Elastomers
Impact ionization
Electrons
Anhydrides
Amides
Carcinogens
Amines
Methanol
Toxicity
Protons
Polymers
Animals
Ions
Detectors
Acids
Fluids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Quantification of 4,4′-diaminodiphenylmethane by gas chromatography negative ion chemical lonization mass spectrometry. / Benfenati, Emilio; Natangelo, Marco; Fanelli, Roberto; Lualdi, Gabriele; Tridico, Rosa.

In: Microchemical Journal, Vol. 46, No. 3, 1992, p. 352-359.

Research output: Contribution to journalArticle

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