Quantification of apoC-II and apoC-III of human very low density lipoproteins by analytical isoelectric focusing

A. L. Catapano, R. L. Jackson, E. B. Gilliam, A. M. Gotto, L. C. Smith

Research output: Contribution to journalArticle

Abstract

ApoC-II and apo-C-III of human very low density lipoproteins (VLDL) have been quantified by analytical isoelectric focusing (IEF) between pH 4 and 6 in polyacrylamide gels containing 8 M urea. The isoelectric point of apoC-III 0 is pH 4.93; apoC-II, pH 4.78; apoC-III 1, pH 4.72, and apoC-III 2, pH 4.54. ApoC-I is not found in the pH range between pH 4 and 6. Two minor peptides, apoC-IV and apoC-V, with isoelectric points of pH 4.61 and 4.44, respectively, are apoproteins not previously identified. The sensitivity (5-40 μg) and reproducibility (±8%) of this method allow quantitative analysis of apoC-II and apoC-III distribution in VLDL.

Original languageEnglish
Pages (from-to)1047-1052
Number of pages6
JournalJournal of Lipid Research
Volume19
Issue number8
Publication statusPublished - 1978

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Quantification of apoC-II and apoC-III of human very low density lipoproteins by analytical isoelectric focusing'. Together they form a unique fingerprint.

  • Cite this