Quantification of arginine and its metabolites in human erythrocytes using liquid chromatography-tandem mass spectrometry

Isabella Squellerio, Elena Tremoli, Viviana Cavalca

Research output: Contribution to journalArticle

Abstract

Erythrocytes may affect several physiological processes because they are scavengers, vehicles, and (as recently highlighted) a producer of nitric oxide (NO). NO bioavailability is linked to arginine, its metabolic products ornithine and citrulline, and methylarginines. Here we describe a liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of analytes involved in the Arg/NO metabolic pathway in erythrocytes. Calibration functions were linear, and the interday coefficients of variation were less than 10%. Limit of quantification values make this method suitable for low concentration samples. The method presented here allows easy sample preparation and provides a valuable tool for the evaluation of the Arg/NO metabolic pathway in erythrocytes.

Original languageEnglish
Pages (from-to)108-110
Number of pages3
JournalAnalytical Biochemistry
Volume412
Issue number1
DOIs
Publication statusPublished - May 1 2011

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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