Quantification of chloride channel 2 (CLCN2) gene isoforms in normal versus lesion- and epilepsy-associated brain tissue

Matteo Bertelli, Stefano Cecchin, Cristina Lapucci, Paola de Gemmis, Daniela Danieli, Emanuele S G d'Amore, Luciano Buttolo, Filippo Giunta, Pietro Mortini, Massimo Pandolfo

Research output: Contribution to journalArticlepeer-review

Abstract

The chloride channel 2 (CLCN2) gene codes for a protein organized in N- and C-terminal regions with regulatory functions and a transmembrane region which forms the ring of the pore. Mutations in the gene have previously been described in patients with idiopathic familial epilepsy. In this study we looked for new isoforms of CLCN2 and we estimated expression levels by real time PCR in brain tissue containing epileptic foci. Samples used in this study were first analyzed and selected to exclude mutations in the coding region of the gene. Four isoforms (skipping exons 3, 16, 22 and 6/7) were identified and quantified by Real Time PCR and compared with total expression of the gene. Expression of the region common to all CLCN2 isoforms was 50% less in epilepsy-associated brain tissue than in controls. The ratio of the various isoforms was slightly greater in epileptic than control tissue. The greatest difference was recorded in the temporal lobe for the isoform with skipped exon 22. Analysis of these isoforms in brain tissue containing epileptic foci suggests that CLCN2 could be implicated in epilepsy, even in the absence of mutations.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1772
Issue number1
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Alternative splicing
  • Chloride Channel 2
  • Epilepsy
  • Real Time PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Biophysics

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