TY - JOUR
T1 - Quantification of piperacillin, tazobactam, meropenem, ceftazidime, and linezolid in human plasma by liquid chromatography/ tandem mass spectrometry
AU - Barco, Sebastiano
AU - Bandettini, Roberto
AU - Maffia, Angelo
AU - Tripodi, Gino
AU - Castagnola, Elio
AU - Cangemi, Giuliana
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Therapeutic drug monitoring is a cornerstone of antibacterial therapy, especially in an era of increasing antibacterial resistance in individualizing antimicrobial therapy. Liquid chromatography/tandem mass spectrometry (LC–MS/MS) assay was used for the simultaneous measurement of piperacillin, tazobactam, meropenem, ceftazidime, and linezolid in 50 ml plasma samples over a wide range. The overall turnaround time for the assay was 20 minutes. Intra-assay precision and accuracy for quality control samples ranged within 1.8–8.5 and 91.4–106.7%, respectively. Inter-assay precision and accuracy ranged within 1.3–14.4 and 95.8–104.6%, respectively. The lower limit of quantification was below 1.5 mg/ml for all the five antibiotics. No ion suppression due to matrix effects was found. A simple and rapid LC–MS/MS method which provides high specificity, precision and accuracy for the simultaneous quantification of piperacillin, tazobactam, meropenem, ceftazidime, and linezolid in human plasma has been developed and validated. The present method is suitable for therapeutic drug monitoring in paediatrics.
AB - Therapeutic drug monitoring is a cornerstone of antibacterial therapy, especially in an era of increasing antibacterial resistance in individualizing antimicrobial therapy. Liquid chromatography/tandem mass spectrometry (LC–MS/MS) assay was used for the simultaneous measurement of piperacillin, tazobactam, meropenem, ceftazidime, and linezolid in 50 ml plasma samples over a wide range. The overall turnaround time for the assay was 20 minutes. Intra-assay precision and accuracy for quality control samples ranged within 1.8–8.5 and 91.4–106.7%, respectively. Inter-assay precision and accuracy ranged within 1.3–14.4 and 95.8–104.6%, respectively. The lower limit of quantification was below 1.5 mg/ml for all the five antibiotics. No ion suppression due to matrix effects was found. A simple and rapid LC–MS/MS method which provides high specificity, precision and accuracy for the simultaneous quantification of piperacillin, tazobactam, meropenem, ceftazidime, and linezolid in human plasma has been developed and validated. The present method is suitable for therapeutic drug monitoring in paediatrics.
KW - Ceftazidime
KW - Linezolid
KW - Liquid chromatography/tandem mass spectrometry
KW - Meropenem
KW - Piperacillin
KW - Tazobactam
UR - http://www.scopus.com/inward/record.url?scp=84954067047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84954067047&partnerID=8YFLogxK
U2 - 10.1179/1973947814Y.0000000209
DO - 10.1179/1973947814Y.0000000209
M3 - Article
AN - SCOPUS:84954067047
VL - 27
SP - 343
EP - 347
JO - Journal of Chemotherapy
JF - Journal of Chemotherapy
SN - 1120-009X
IS - 6
ER -