Quantification of small non-coding RNAs allows an accurate comparison of miRNA expression profiles

Andrea Masotti, Viviana Caputo, Letizia Da Sacco, Antonio Pizzuti, Bruno Dallapiccola, Gian Franco Bottazzo

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs) are highly conserved 22-mer RNA molecules, encoded by plants and animals that regulate the expression of genes binding to the 3 ′ -UTR of specific target mRNAs. The amount of miRNAs in a total RNA sample depends on the recovery efficiency that may be significantly affected by the different purification methods employed. Traditional approaches may be inefficient at recovering small RNAs, and common spectrophotometric determination is not adequate to quantify selectively these low molecular weight (LMW) species from total RNA samples. Here, we describe the use of qualitative and quantitative lab-on-a-chip tools for the analysis of these LMW RNA species. Our data emphasize the close correlation of LMW RNAs with the expression levels of some miRNAs. We therefore applied our result to the comparison of some miRNA expression profiles in different tissues. Finally, the methods we used in this paper allowed us to analyze the efficiency of extraction protocols, to study the small (but significant) differences among various preparations and to allow a proper comparison of some miRNA expression profiles in various tissues.

Original languageEnglish
Article number659028
JournalJournal of Biomedicine and Biotechnology
Volume2009
DOIs
Publication statusPublished - 2009

Fingerprint

Small Untranslated RNA
MicroRNAs
RNA
Molecular Weight
3' Untranslated Regions
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Quantification of small non-coding RNAs allows an accurate comparison of miRNA expression profiles. / Masotti, Andrea; Caputo, Viviana; Da Sacco, Letizia; Pizzuti, Antonio; Dallapiccola, Bruno; Bottazzo, Gian Franco.

In: Journal of Biomedicine and Biotechnology, Vol. 2009, 659028, 2009.

Research output: Contribution to journalArticle

@article{f2465bda8af54ca8aab7e4e9ce068c0c,
title = "Quantification of small non-coding RNAs allows an accurate comparison of miRNA expression profiles",
abstract = "MicroRNAs (miRNAs) are highly conserved 22-mer RNA molecules, encoded by plants and animals that regulate the expression of genes binding to the 3 ′ -UTR of specific target mRNAs. The amount of miRNAs in a total RNA sample depends on the recovery efficiency that may be significantly affected by the different purification methods employed. Traditional approaches may be inefficient at recovering small RNAs, and common spectrophotometric determination is not adequate to quantify selectively these low molecular weight (LMW) species from total RNA samples. Here, we describe the use of qualitative and quantitative lab-on-a-chip tools for the analysis of these LMW RNA species. Our data emphasize the close correlation of LMW RNAs with the expression levels of some miRNAs. We therefore applied our result to the comparison of some miRNA expression profiles in different tissues. Finally, the methods we used in this paper allowed us to analyze the efficiency of extraction protocols, to study the small (but significant) differences among various preparations and to allow a proper comparison of some miRNA expression profiles in various tissues.",
author = "Andrea Masotti and Viviana Caputo and {Da Sacco}, Letizia and Antonio Pizzuti and Bruno Dallapiccola and Bottazzo, {Gian Franco}",
year = "2009",
doi = "10.1155/2009/659028",
language = "English",
volume = "2009",
journal = "Journal of Biomedicine and Biotechnology",
issn = "1110-7243",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Quantification of small non-coding RNAs allows an accurate comparison of miRNA expression profiles

AU - Masotti, Andrea

AU - Caputo, Viviana

AU - Da Sacco, Letizia

AU - Pizzuti, Antonio

AU - Dallapiccola, Bruno

AU - Bottazzo, Gian Franco

PY - 2009

Y1 - 2009

N2 - MicroRNAs (miRNAs) are highly conserved 22-mer RNA molecules, encoded by plants and animals that regulate the expression of genes binding to the 3 ′ -UTR of specific target mRNAs. The amount of miRNAs in a total RNA sample depends on the recovery efficiency that may be significantly affected by the different purification methods employed. Traditional approaches may be inefficient at recovering small RNAs, and common spectrophotometric determination is not adequate to quantify selectively these low molecular weight (LMW) species from total RNA samples. Here, we describe the use of qualitative and quantitative lab-on-a-chip tools for the analysis of these LMW RNA species. Our data emphasize the close correlation of LMW RNAs with the expression levels of some miRNAs. We therefore applied our result to the comparison of some miRNA expression profiles in different tissues. Finally, the methods we used in this paper allowed us to analyze the efficiency of extraction protocols, to study the small (but significant) differences among various preparations and to allow a proper comparison of some miRNA expression profiles in various tissues.

AB - MicroRNAs (miRNAs) are highly conserved 22-mer RNA molecules, encoded by plants and animals that regulate the expression of genes binding to the 3 ′ -UTR of specific target mRNAs. The amount of miRNAs in a total RNA sample depends on the recovery efficiency that may be significantly affected by the different purification methods employed. Traditional approaches may be inefficient at recovering small RNAs, and common spectrophotometric determination is not adequate to quantify selectively these low molecular weight (LMW) species from total RNA samples. Here, we describe the use of qualitative and quantitative lab-on-a-chip tools for the analysis of these LMW RNA species. Our data emphasize the close correlation of LMW RNAs with the expression levels of some miRNAs. We therefore applied our result to the comparison of some miRNA expression profiles in different tissues. Finally, the methods we used in this paper allowed us to analyze the efficiency of extraction protocols, to study the small (but significant) differences among various preparations and to allow a proper comparison of some miRNA expression profiles in various tissues.

UR - http://www.scopus.com/inward/record.url?scp=70349257339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349257339&partnerID=8YFLogxK

U2 - 10.1155/2009/659028

DO - 10.1155/2009/659028

M3 - Article

C2 - 19727414

AN - SCOPUS:70349257339

VL - 2009

JO - Journal of Biomedicine and Biotechnology

JF - Journal of Biomedicine and Biotechnology

SN - 1110-7243

M1 - 659028

ER -