A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 102 CFU/μl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo.
- Bacillus clausii
- Real-time PCR
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology