Quantitation of Bacillus clausii in biological samples by real-time polymerase chain reaction

Mario Perotti, Nicasio Mancini, Annalisa Cavallero, Silvia Carletti, Filippo Canducci, Roberto Burioni, Massimo Clementi

Research output: Contribution to journalArticle

Abstract

A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 102 CFU/μl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo.

Original languageEnglish
Pages (from-to)632-636
Number of pages5
JournalJournal of Microbiological Methods
Volume65
Issue number3
DOIs
Publication statusPublished - Jun 2006

Keywords

  • Bacillus clausii
  • Probiotics
  • Quantitation
  • Real-time PCR

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

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