TY - JOUR
T1 - Quantitation of biologically active IL-1 by a sensitive assay based on immobilized human IL-1 receptor type II (IL-1RII)
AU - Scapigliati, Giuseppe
AU - Bossu, Paola
AU - Censini, Stefano
AU - Tagliabue, Aldo
AU - Boraschi, Diana
AU - Ghiara, Paolo
PY - 1991/4/8
Y1 - 1991/4/8
N2 - A rapid and sensitive solid-phase radioassay is described for the quantitative detection of human interleukin-1 (IL-1) based on its capability to bind the nitrocellulose-immobilized IL-1 receptor solubilized from plasma membranes of a subclone of the human B cell lymphoma Raji. The assay can detect human IL-1β levels as low as 1 × 10-11 M, both in physiological buffers and in human plasma. Much lower sensitivity was observed for human IL-1α (3.7 × 10-9 M) and murine IL-1β (2 × 10-9 M). This assay has the advantage to specifically detect only the correctly folded biologically active IL-1. Simple pretreatment procedure that selectively removes IL-1β from samples has been devised so that the ratio of the two IL-1s isoforms in the sample can be precisely determined. This assay represents a fast method for the simultaneous-testing of large numbers of biological samples.
AB - A rapid and sensitive solid-phase radioassay is described for the quantitative detection of human interleukin-1 (IL-1) based on its capability to bind the nitrocellulose-immobilized IL-1 receptor solubilized from plasma membranes of a subclone of the human B cell lymphoma Raji. The assay can detect human IL-1β levels as low as 1 × 10-11 M, both in physiological buffers and in human plasma. Much lower sensitivity was observed for human IL-1α (3.7 × 10-9 M) and murine IL-1β (2 × 10-9 M). This assay has the advantage to specifically detect only the correctly folded biologically active IL-1. Simple pretreatment procedure that selectively removes IL-1β from samples has been devised so that the ratio of the two IL-1s isoforms in the sample can be precisely determined. This assay represents a fast method for the simultaneous-testing of large numbers of biological samples.
KW - Interleukin-1 radioassay
KW - Interleukin-1 receptor
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U2 - 10.1016/0022-1759(91)90061-J
DO - 10.1016/0022-1759(91)90061-J
M3 - Article
C2 - 1826916
AN - SCOPUS:0025853579
VL - 138
SP - 31
EP - 38
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1
ER -