Quantitation of biologically active IL-1 by a sensitive assay based on immobilized human IL-1 receptor type II (IL-1RII)

Giuseppe Scapigliati, Paola Bossu, Stefano Censini, Aldo Tagliabue, Diana Boraschi, Paolo Ghiara

Research output: Contribution to journalArticlepeer-review

Abstract

A rapid and sensitive solid-phase radioassay is described for the quantitative detection of human interleukin-1 (IL-1) based on its capability to bind the nitrocellulose-immobilized IL-1 receptor solubilized from plasma membranes of a subclone of the human B cell lymphoma Raji. The assay can detect human IL-1β levels as low as 1 × 10-11 M, both in physiological buffers and in human plasma. Much lower sensitivity was observed for human IL-1α (3.7 × 10-9 M) and murine IL-1β (2 × 10-9 M). This assay has the advantage to specifically detect only the correctly folded biologically active IL-1. Simple pretreatment procedure that selectively removes IL-1β from samples has been devised so that the ratio of the two IL-1s isoforms in the sample can be precisely determined. This assay represents a fast method for the simultaneous-testing of large numbers of biological samples.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalJournal of Immunological Methods
Volume138
Issue number1
DOIs
Publication statusPublished - Apr 8 1991

Keywords

  • Interleukin-1 radioassay
  • Interleukin-1 receptor

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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