Quantitation of biologically active IL-1 by a sensitive assay based on immobilized human IL-1 receptor type II (IL-1RII)

Giuseppe Scapigliati; Paola Bossu; Stefano Censini; Aldo Tagliabue; Diana Boraschi; Paolo Ghiara

doi:10.1016/0022-1759(91)90061-J

Quantitation of biologically active IL-1 by a sensitive assay based on immobilized human IL-1 receptor type II (IL-1RII)

Giuseppe Scapigliati, Paola Bossu, Stefano Censini, Aldo Tagliabue, Diana Boraschi, Paolo Ghiara

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid and sensitive solid-phase radioassay is described for the quantitative detection of human interleukin-1 (IL-1) based on its capability to bind the nitrocellulose-immobilized IL-1 receptor solubilized from plasma membranes of a subclone of the human B cell lymphoma Raji. The assay can detect human IL-1β levels as low as 1 × 10^-11 M, both in physiological buffers and in human plasma. Much lower sensitivity was observed for human IL-1α (3.7 × 10^-9 M) and murine IL-1β (2 × 10^-9 M). This assay has the advantage to specifically detect only the correctly folded biologically active IL-1. Simple pretreatment procedure that selectively removes IL-1β from samples has been devised so that the ratio of the two IL-1s isoforms in the sample can be precisely determined. This assay represents a fast method for the simultaneous-testing of large numbers of biological samples.

Original language	English
Pages (from-to)	31-38
Number of pages	8
Journal	Journal of Immunological Methods
Volume	138
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(91)90061-J
Publication status	Published - Apr 8 1991

Keywords

Interleukin-1 radioassay
Interleukin-1 receptor

ASJC Scopus subject areas

Biotechnology
Immunology

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Quantitation of biologically active IL-1 by a sensitive assay based on immobilized human IL-1 receptor type II (IL-1RII). / Scapigliati, Giuseppe; Bossu, Paola; Censini, Stefano; Tagliabue, Aldo; Boraschi, Diana; Ghiara, Paolo.

In: Journal of Immunological Methods, Vol. 138, No. 1, 08.04.1991, p. 31-38.

Research output: Contribution to journal › Article › peer-review

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abstract = "A rapid and sensitive solid-phase radioassay is described for the quantitative detection of human interleukin-1 (IL-1) based on its capability to bind the nitrocellulose-immobilized IL-1 receptor solubilized from plasma membranes of a subclone of the human B cell lymphoma Raji. The assay can detect human IL-1β levels as low as 1 × 10-11 M, both in physiological buffers and in human plasma. Much lower sensitivity was observed for human IL-1α (3.7 × 10-9 M) and murine IL-1β (2 × 10-9 M). This assay has the advantage to specifically detect only the correctly folded biologically active IL-1. Simple pretreatment procedure that selectively removes IL-1β from samples has been devised so that the ratio of the two IL-1s isoforms in the sample can be precisely determined. This assay represents a fast method for the simultaneous-testing of large numbers of biological samples.",

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AU - Ghiara, Paolo

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