TY - JOUR
T1 - Quantitative analyses of plasma opsonizing activity and polymorphonuclear cell response during phagocytosis
T2 - Standardisation of a chemiluminescent method
AU - Farinelli, Andrea
AU - Setti, Maurizio
AU - Puppo, Francesco
AU - Scudeletti, Marco
AU - De Martini, Daniele
AU - Balestra, Vincenzo
AU - Indiveri, Francesco
PY - 1996
Y1 - 1996
N2 - Although measurement of chemiluminescence has become a widespread tool in the study of phagocytosis of peripheral neutrophils, several problems linked to spontaneous fluctuation in chemiluminescence and the number of variables involved have occasionally either limited its usefulness for clinical and experimental purposes or compelled operators to take particular care when using the technique. In the present paper, sources of variability are investigated and most of the parameters involved are thoroughly analysed and step-by-step normalised. A stochastic calibration procedure for validation of the method is applied and a monofunctional test protocol for quantitative evaluation of plasma opsonizing activity in whole blood chemiluminescence is suggested. With regard to the goal of proposing a reverse monofunctional test, we discuss the reasons why further studies aimed at standardised evaluation of the cellular components are needed.
AB - Although measurement of chemiluminescence has become a widespread tool in the study of phagocytosis of peripheral neutrophils, several problems linked to spontaneous fluctuation in chemiluminescence and the number of variables involved have occasionally either limited its usefulness for clinical and experimental purposes or compelled operators to take particular care when using the technique. In the present paper, sources of variability are investigated and most of the parameters involved are thoroughly analysed and step-by-step normalised. A stochastic calibration procedure for validation of the method is applied and a monofunctional test protocol for quantitative evaluation of plasma opsonizing activity in whole blood chemiluminescence is suggested. With regard to the goal of proposing a reverse monofunctional test, we discuss the reasons why further studies aimed at standardised evaluation of the cellular components are needed.
KW - Aniinfective immunity
KW - Immunological methods
KW - Neutrophil functions
KW - Phagocytosis
KW - Whole blood chemiluminescence
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M3 - Article
C2 - 8920802
AN - SCOPUS:0029989359
VL - 104
SP - 500
EP - 508
JO - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section B Microbiology
JF - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section B Microbiology
SN - 0365-5555
IS - 7-8
ER -