Quantitative analyses of plasma opsonizing activity and polymorphonuclear cell response during phagocytosis: Standardisation of a chemiluminescent method

Andrea Farinelli, Maurizio Setti, Francesco Puppo, Marco Scudeletti, Daniele De Martini, Vincenzo Balestra, Francesco Indiveri

Research output: Contribution to journalArticle

Abstract

Although measurement of chemiluminescence has become a widespread tool in the study of phagocytosis of peripheral neutrophils, several problems linked to spontaneous fluctuation in chemiluminescence and the number of variables involved have occasionally either limited its usefulness for clinical and experimental purposes or compelled operators to take particular care when using the technique. In the present paper, sources of variability are investigated and most of the parameters involved are thoroughly analysed and step-by-step normalised. A stochastic calibration procedure for validation of the method is applied and a monofunctional test protocol for quantitative evaluation of plasma opsonizing activity in whole blood chemiluminescence is suggested. With regard to the goal of proposing a reverse monofunctional test, we discuss the reasons why further studies aimed at standardised evaluation of the cellular components are needed.

Original languageEnglish
Pages (from-to)500-508
Number of pages9
JournalAPMIS
Volume104
Issue number7-8
Publication statusPublished - 1996

Keywords

  • Aniinfective immunity
  • Immunological methods
  • Neutrophil functions
  • Phagocytosis
  • Whole blood chemiluminescence

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Pathology and Forensic Medicine

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    Farinelli, A., Setti, M., Puppo, F., Scudeletti, M., De Martini, D., Balestra, V., & Indiveri, F. (1996). Quantitative analyses of plasma opsonizing activity and polymorphonuclear cell response during phagocytosis: Standardisation of a chemiluminescent method. APMIS, 104(7-8), 500-508.