Quantitative and qualitative analysis of tumor-associated CD4+ T cells

Giulia Di Lullo; Lucia De Monte; Claudio Doglioni; Maria Pia Protti

doi:10.1007/978-1-4939-3338-9_4

Quantitative and qualitative analysis of tumor-associated CD4⁺ T cells

Giulia Di Lullo, Lucia De Monte, Claudio Doglioni, Maria Pia Protti

IRCCS Ospedale San Raffaele

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

CD4⁺ T cells comprise a significant portion of tumor-infiltrating lymphocytes. Different subsets of CD4⁺ T cells exist they exert different effector functions in tumor immunity depending on the cytokines produced going from antitumor to pro-tumor. Methods that use small aliquots of cells to identify ex vivo the frequency and functional orientation of tumor-specific CD4⁺ T cells in the blood and visualization of the presence of different CD4⁺ T cell subsets and their localization at the tumor site are valuable tools to determine their clinical impact in neoplastic diseases.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	37-51
Number of pages	15
Volume	1393
DOIs	https://doi.org/10.1007/978-1-4939-3338-9_4
Publication status	Published - Apr 1 2016

Publication series

Name	Methods in Molecular Biology
Volume	1393
ISSN (Print)	10643745

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Keywords

Antigen-specific CD4 T cell frequency
Antitumor cytokine
CD4 T cells
GATA-3
Pro-tumor cytokine
T-bet
Th subsets
Tumor antigens

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Di Lullo, G., De Monte, L., Doglioni, C., & Protti, M. P. (2016). Quantitative and qualitative analysis of tumor-associated CD4⁺ T cells. In Methods in Molecular Biology (Vol. 1393, pp. 37-51). (Methods in Molecular Biology; Vol. 1393). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3338-9_4

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Access to Document

10.1007/978-1-4939-3338-9_4