Quantitative and qualitative analysis of tumor-associated CD4+ T cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

CD4+ T cells comprise a significant portion of tumor-infiltrating lymphocytes. Different subsets of CD4+ T cells exist they exert different effector functions in tumor immunity depending on the cytokines produced going from antitumor to pro-tumor. Methods that use small aliquots of cells to identify ex vivo the frequency and functional orientation of tumor-specific CD4+ T cells in the blood and visualization of the presence of different CD4+ T cell subsets and their localization at the tumor site are valuable tools to determine their clinical impact in neoplastic diseases.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages37-51
Number of pages15
Volume1393
DOIs
Publication statusPublished - Apr 1 2016

Publication series

NameMethods in Molecular Biology
Volume1393
ISSN (Print)10643745

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Keywords

  • Antigen-specific CD4 T cell frequency
  • Antitumor cytokine
  • CD4 T cells
  • GATA-3
  • Pro-tumor cytokine
  • T-bet
  • Th subsets
  • Tumor antigens

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Di Lullo, G., De Monte, L., Doglioni, C., & Protti, M. P. (2016). Quantitative and qualitative analysis of tumor-associated CD4+ T cells. In Methods in Molecular Biology (Vol. 1393, pp. 37-51). (Methods in Molecular Biology; Vol. 1393). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3338-9_4