Quantitative and qualitative analysis of tumor-associated CD4+ T cells

Giulia Di Lullo, Lucia De Monte, Claudio Doglioni, Maria Pia Protti

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

CD4+ T cells comprise a significant portion of tumor-infiltrating lymphocytes. Different subsets of CD4+ T cells exist they exert different effector functions in tumor immunity depending on the cytokines produced going from antitumor to pro-tumor. Methods that use small aliquots of cells to identify ex vivo the frequency and functional orientation of tumor-specific CD4+ T cells in the blood and visualization of the presence of different CD4+ T cell subsets and their localization at the tumor site are valuable tools to determine their clinical impact in neoplastic diseases.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages37-51
Number of pages15
Volume1393
DOIs
Publication statusPublished - Apr 1 2016

Publication series

NameMethods in Molecular Biology
Volume1393
ISSN (Print)10643745

Fingerprint

T-Lymphocytes
T-Lymphocyte Subsets
Neoplasms
Tumor-Infiltrating Lymphocytes
Immunity
Cytokines

Keywords

  • Antigen-specific CD4 T cell frequency
  • Antitumor cytokine
  • CD4 T cells
  • GATA-3
  • Pro-tumor cytokine
  • T-bet
  • Th subsets
  • Tumor antigens

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Di Lullo, G., De Monte, L., Doglioni, C., & Protti, M. P. (2016). Quantitative and qualitative analysis of tumor-associated CD4+ T cells. In Methods in Molecular Biology (Vol. 1393, pp. 37-51). (Methods in Molecular Biology; Vol. 1393). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3338-9_4

Quantitative and qualitative analysis of tumor-associated CD4+ T cells. / Di Lullo, Giulia; De Monte, Lucia; Doglioni, Claudio; Protti, Maria Pia.

Methods in Molecular Biology. Vol. 1393 Humana Press Inc., 2016. p. 37-51 (Methods in Molecular Biology; Vol. 1393).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Di Lullo, G, De Monte, L, Doglioni, C & Protti, MP 2016, Quantitative and qualitative analysis of tumor-associated CD4+ T cells. in Methods in Molecular Biology. vol. 1393, Methods in Molecular Biology, vol. 1393, Humana Press Inc., pp. 37-51. https://doi.org/10.1007/978-1-4939-3338-9_4
Di Lullo G, De Monte L, Doglioni C, Protti MP. Quantitative and qualitative analysis of tumor-associated CD4+ T cells. In Methods in Molecular Biology. Vol. 1393. Humana Press Inc. 2016. p. 37-51. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3338-9_4
Di Lullo, Giulia ; De Monte, Lucia ; Doglioni, Claudio ; Protti, Maria Pia. / Quantitative and qualitative analysis of tumor-associated CD4+ T cells. Methods in Molecular Biology. Vol. 1393 Humana Press Inc., 2016. pp. 37-51 (Methods in Molecular Biology).
@inbook{1ea3d2c385574298b57c33900687c9f8,
title = "Quantitative and qualitative analysis of tumor-associated CD4+ T cells",
abstract = "CD4+ T cells comprise a significant portion of tumor-infiltrating lymphocytes. Different subsets of CD4+ T cells exist they exert different effector functions in tumor immunity depending on the cytokines produced going from antitumor to pro-tumor. Methods that use small aliquots of cells to identify ex vivo the frequency and functional orientation of tumor-specific CD4+ T cells in the blood and visualization of the presence of different CD4+ T cell subsets and their localization at the tumor site are valuable tools to determine their clinical impact in neoplastic diseases.",
keywords = "Antigen-specific CD4 T cell frequency, Antitumor cytokine, CD4 T cells, GATA-3, Pro-tumor cytokine, T-bet, Th subsets, Tumor antigens",
author = "{Di Lullo}, Giulia and {De Monte}, Lucia and Claudio Doglioni and Protti, {Maria Pia}",
year = "2016",
month = "4",
day = "1",
doi = "10.1007/978-1-4939-3338-9_4",
language = "English",
volume = "1393",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "37--51",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Quantitative and qualitative analysis of tumor-associated CD4+ T cells

AU - Di Lullo, Giulia

AU - De Monte, Lucia

AU - Doglioni, Claudio

AU - Protti, Maria Pia

PY - 2016/4/1

Y1 - 2016/4/1

N2 - CD4+ T cells comprise a significant portion of tumor-infiltrating lymphocytes. Different subsets of CD4+ T cells exist they exert different effector functions in tumor immunity depending on the cytokines produced going from antitumor to pro-tumor. Methods that use small aliquots of cells to identify ex vivo the frequency and functional orientation of tumor-specific CD4+ T cells in the blood and visualization of the presence of different CD4+ T cell subsets and their localization at the tumor site are valuable tools to determine their clinical impact in neoplastic diseases.

AB - CD4+ T cells comprise a significant portion of tumor-infiltrating lymphocytes. Different subsets of CD4+ T cells exist they exert different effector functions in tumor immunity depending on the cytokines produced going from antitumor to pro-tumor. Methods that use small aliquots of cells to identify ex vivo the frequency and functional orientation of tumor-specific CD4+ T cells in the blood and visualization of the presence of different CD4+ T cell subsets and their localization at the tumor site are valuable tools to determine their clinical impact in neoplastic diseases.

KW - Antigen-specific CD4 T cell frequency

KW - Antitumor cytokine

KW - CD4 T cells

KW - GATA-3

KW - Pro-tumor cytokine

KW - T-bet

KW - Th subsets

KW - Tumor antigens

UR - http://www.scopus.com/inward/record.url?scp=84962719631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962719631&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3338-9_4

DO - 10.1007/978-1-4939-3338-9_4

M3 - Chapter

VL - 1393

T3 - Methods in Molecular Biology

SP - 37

EP - 51

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Access to Document