@inbook{1ea3d2c385574298b57c33900687c9f8,
title = "Quantitative and qualitative analysis of tumor-associated CD4+ T cells",
abstract = "CD4+ T cells comprise a significant portion of tumor-infiltrating lymphocytes. Different subsets of CD4+ T cells exist they exert different effector functions in tumor immunity depending on the cytokines produced going from antitumor to pro-tumor. Methods that use small aliquots of cells to identify ex vivo the frequency and functional orientation of tumor-specific CD4+ T cells in the blood and visualization of the presence of different CD4+ T cell subsets and their localization at the tumor site are valuable tools to determine their clinical impact in neoplastic diseases.",
keywords = "Antigen-specific CD4 T cell frequency, Antitumor cytokine, CD4 T cells, GATA-3, Pro-tumor cytokine, T-bet, Th subsets, Tumor antigens",
author = "{Di Lullo}, Giulia and {De Monte}, Lucia and Claudio Doglioni and Protti, {Maria Pia}",
year = "2016",
month = apr,
day = "1",
doi = "10.1007/978-1-4939-3338-9_4",
language = "English",
volume = "1393",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "37--51",
booktitle = "Methods in Molecular Biology",
}