Quantitative and qualitative characterization of plasma DNA identifies primary and recurrent colorectal cancer

M. Frattini, G. Gallino, S. Signoroni, D. Balestra, L. Lusa, L. Battaglia, G. Sozzi, L. Bertario, E. Leo, S. Pilotti, M. A. Pierotti

Research output: Contribution to journalArticlepeer-review

Abstract

Because plasma DNA may be a useful tool for cancer detection, we screened primary tumors and related multiple plasma samples at the time of surgery and during the follow-up period for plasma DNA level as well as for K-Ras mutations and p16INK4a promoter hypermethylation in colorectal cancer patients. At the time of surgery, DNA levels were higher in tumor patients than in healthy donors, and K-Ras and p16INK4a alterations were detected in 7 and 11 cancers respectively, and in all related plasma samples. During the follow-up, plasma DNA levels decrease progressively but rapidly increased when a relapse occurred, whereas K-Ras and p16INK4a alterations were detected only in relapsed patients. Therefore, combined quantitative and qualitative analyses of plasma DNA confirm the presence of colorectal cancer, define disease-free status and indicate the presence of relapse.

Original languageEnglish
Pages (from-to)170-181
Number of pages12
JournalCancer Letters
Volume263
Issue number2
DOIs
Publication statusPublished - May 18 2008

Keywords

  • Colorectal cancer
  • K-Ras
  • p16
  • Plasma
  • Quantitative analysis

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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