Quantitative automated particle-enhanced immunonephelometric assay for the routinary measurement of human cystatin C

Michele Mussap, Nicoletta Ruzzante, Mariacristina Varagnolo, Mario Plebani

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Human cystatin C is a low molecular mass protein of 13359 Dalton recently proposed as a new very sensitive marker of changes in glomerular filtration rate. Serum cystatin C concentration correlates negatively with glomerular filtration rate as well as or better than creatinine. We evaluated a recently introduced automated nephelometric immunoassay for cystatin C in serum or EDTA-plasma samples on the Behring Nephelometer System. The assay consists of incubating the 100-fold diluted sample for 6 minutes with latex particles covalently coated with anti-human cystatin C antibodies, and then quantifying the change of light-scatter produced. Method reproducibility is satisfactory, the intra- and inter-assay coefficients of variation ranging from 1.58% to 3.77% and from 5.6% to 11.47% respectively. Rheumatoid factor (≤ 1116 IU/ml), bilirubin (≤ 418 μmmol/l), triglycerides (10.47 mmol/l), and haemoglobin (12 g/l) do not significantly interfere in the assay. No significant difference was found in cystatin C concentration between serum and EDTA-plasma samples. Cystatin C is stable in serum samples stored under different conditions up to one month. This method correlates well (mean difference = -0.536 ± 0.307 mg/l) with another commercially available particle-enhanced turbidimetric immunoassay. Cystatin C offers better clinical sensitivity than creatinine for discriminating patients with normal renal function and those with mild-to-moderate reduction in renal function. This method is suitable for routine cystatin C measurement, including emergencies.

Original languageEnglish
Pages (from-to)859-865
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Volume36
Issue number11
Publication statusPublished - 1998

Fingerprint

Cystatin C
Assays
Serum
Glomerular Filtration Rate
Immunoassay
Edetic Acid
Creatinine
Nephelometers
Kidney
Plasmas
Rheumatoid Factor
Latex
Molecular mass
Microspheres
Bilirubin
Hemoglobins
Triglycerides
Emergencies
Light
Antibodies

Keywords

  • Cystatin C
  • Glomerular filtration rate
  • Latex immunoassay
  • Nephelometry
  • Renal function

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Quantitative automated particle-enhanced immunonephelometric assay for the routinary measurement of human cystatin C. / Mussap, Michele; Ruzzante, Nicoletta; Varagnolo, Mariacristina; Plebani, Mario.

In: Clinical Chemistry and Laboratory Medicine, Vol. 36, No. 11, 1998, p. 859-865.

Research output: Contribution to journalArticle

Mussap, Michele ; Ruzzante, Nicoletta ; Varagnolo, Mariacristina ; Plebani, Mario. / Quantitative automated particle-enhanced immunonephelometric assay for the routinary measurement of human cystatin C. In: Clinical Chemistry and Laboratory Medicine. 1998 ; Vol. 36, No. 11. pp. 859-865.
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