Abstract
Mass fragmentography (MF) and high resolution gas chromatography with electron capture detection (HRGC-ECD) were used for measuring 6-keto-PGF(1α) the stable hydrolysis product of prostacyclin (PGI2) released by fresh rings of rat aorta, incubated in the absence of the precursors arachidonic acid or prostaglandin endoperaxide (PGH2). The incubation medium was acidified, extracted, chromatographed on silicic acid column and derivatized. Comparable results were obtained analyzing each sample by MF and HRGC-ECD. Both methods proved to be suitable in terms of sensitivity and specificity for the measurement of 6-keto-PGF(1α) produced by individual rat aortae.
| Original language | English |
|---|---|
| Pages (from-to) | 747-758 |
| Number of pages | 12 |
| Journal | Prostaglandins |
| Volume | 20 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1980 |
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ASJC Scopus subject areas
- Biochemistry
- Endocrinology
Cite this
Quantitative determination of 6-keto-PGF1α released by rat aorta : comparison of mass fragmentography and high resolution gas chromatography with electron capture detection. / Chiabrando, C.; Noseda, A.; Noe, M. A.; Fanelli, R.
In: Prostaglandins, Vol. 20, No. 4, 1980, p. 747-758.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative determination of 6-keto-PGF1α released by rat aorta
T2 - comparison of mass fragmentography and high resolution gas chromatography with electron capture detection
AU - Chiabrando, C.
AU - Noseda, A.
AU - Noe, M. A.
AU - Fanelli, R.
PY - 1980
Y1 - 1980
N2 - Mass fragmentography (MF) and high resolution gas chromatography with electron capture detection (HRGC-ECD) were used for measuring 6-keto-PGF(1α) the stable hydrolysis product of prostacyclin (PGI2) released by fresh rings of rat aorta, incubated in the absence of the precursors arachidonic acid or prostaglandin endoperaxide (PGH2). The incubation medium was acidified, extracted, chromatographed on silicic acid column and derivatized. Comparable results were obtained analyzing each sample by MF and HRGC-ECD. Both methods proved to be suitable in terms of sensitivity and specificity for the measurement of 6-keto-PGF(1α) produced by individual rat aortae.
AB - Mass fragmentography (MF) and high resolution gas chromatography with electron capture detection (HRGC-ECD) were used for measuring 6-keto-PGF(1α) the stable hydrolysis product of prostacyclin (PGI2) released by fresh rings of rat aorta, incubated in the absence of the precursors arachidonic acid or prostaglandin endoperaxide (PGH2). The incubation medium was acidified, extracted, chromatographed on silicic acid column and derivatized. Comparable results were obtained analyzing each sample by MF and HRGC-ECD. Both methods proved to be suitable in terms of sensitivity and specificity for the measurement of 6-keto-PGF(1α) produced by individual rat aortae.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019203683&partnerID=8YFLogxK
U2 - 10.1016/0090-6980(80)90113-6
DO - 10.1016/0090-6980(80)90113-6
M3 - Article
VL - 20
SP - 747
EP - 758
JO - Prostaglandins and Other Lipid Mediators
JF - Prostaglandins and Other Lipid Mediators
SN - 1098-8823
IS - 4
ER -