Valutazione mediante analisi d'immagine delle modificazioni morfologiche delle piastrine dopo contatto con polivinilacetato

Translated title of the contribution: Quantitative evaluation by image analysis of platelet morphological modifications after contact with polyvinylacetate

E. Cenni, S. Stea, M. Cervellati, A. Pizzoferrato, L. Montanaro

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. In order to investigate platelet activation after contact with artificial materials, which is an important aspect of biocompatibility especially for the blood-contacting devices, platelet morphological modifications and spread area were evaluated by light microscopy and image analysis after contact with glow discharge-treated polybutylene terephthalate coated with a polymer for platelet concentrate filtration. Methods. A hydrophilic polymer made of partially hydrolyzed polyvinyl-acetate containing polyethylene oxide/poly-propylene oxide copolymer block as lateral chains (PVA) (Biofil S.r.l., Cavezzo, Modena, Italy) was evaluated. After contact with PVA, platelets were allowed to settle on a siliconized slide and then fixed and stained. The specimens were analyzed by image analysis. The percentages of spreading, round and dendritic shapes, as well as the presence of aggregates, were evaluated, and the mean area of the spread platelets was measured. Results. PVA induced significant variations neither in the percentages of shape change distribution, nor of the mean spread area. However it determined a statistically significant reduction in platelets with the area from 60 to 70μ2. Such minimal variations agree with the results we obtained in the past, namely a non significant platelet adhesion induced by the same material. Conclusions. The method confirms the results of platelet adhesion and release reaction (the study of release reaction needs more refined but more expensive methods). However, the study of morphological modifications by image analysis is not suitable for testing materials that induce massive platelet adhesion, because the number of the residual platelets could be too low for the microscopic evaluation.

Original languageItalian
Pages (from-to)1-8
Number of pages8
JournalMinerva Cardioangiologica
Volume48
Issue number1-2
Publication statusPublished - Jan 2000

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Blood Platelets
Polymers
Materials Testing
Platelet Activation
Platelet Count
Italy
Microscopy
Light
Equipment and Supplies

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Valutazione mediante analisi d'immagine delle modificazioni morfologiche delle piastrine dopo contatto con polivinilacetato. / Cenni, E.; Stea, S.; Cervellati, M.; Pizzoferrato, A.; Montanaro, L.

In: Minerva Cardioangiologica, Vol. 48, No. 1-2, 01.2000, p. 1-8.

Research output: Contribution to journalArticle

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abstract = "Background. In order to investigate platelet activation after contact with artificial materials, which is an important aspect of biocompatibility especially for the blood-contacting devices, platelet morphological modifications and spread area were evaluated by light microscopy and image analysis after contact with glow discharge-treated polybutylene terephthalate coated with a polymer for platelet concentrate filtration. Methods. A hydrophilic polymer made of partially hydrolyzed polyvinyl-acetate containing polyethylene oxide/poly-propylene oxide copolymer block as lateral chains (PVA) (Biofil S.r.l., Cavezzo, Modena, Italy) was evaluated. After contact with PVA, platelets were allowed to settle on a siliconized slide and then fixed and stained. The specimens were analyzed by image analysis. The percentages of spreading, round and dendritic shapes, as well as the presence of aggregates, were evaluated, and the mean area of the spread platelets was measured. Results. PVA induced significant variations neither in the percentages of shape change distribution, nor of the mean spread area. However it determined a statistically significant reduction in platelets with the area from 60 to 70μ2. Such minimal variations agree with the results we obtained in the past, namely a non significant platelet adhesion induced by the same material. Conclusions. The method confirms the results of platelet adhesion and release reaction (the study of release reaction needs more refined but more expensive methods). However, the study of morphological modifications by image analysis is not suitable for testing materials that induce massive platelet adhesion, because the number of the residual platelets could be too low for the microscopic evaluation.",
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AU - Pizzoferrato, A.

AU - Montanaro, L.

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