Quantitative evaluation of BCR-ABL amount of transcript post mobilization with G-CSF of peripheral blood stem cells from chronic myeloid leukemia patients in cytogenetic response

G. Martinelli, V. Montefusco, M. Amabile, R. M. Lemoli, C. Terragna, N. Testoni, E. Ottaviani, G. Rosti, A. De Vivo, S. Rizzi, D. Russo, M. Bregoli, S. Tura

Research output: Contribution to journalArticle

Abstract

We studied nine patients affected by chronic myeloid leukemia (CML Ph+ and bcr-abl positive) and treated with alpha-interferon (α-INF) in order to: first, to evaluate the feasibility of a mobilization of peripheral blood stem cells induced by granulocyte-colony-stimulating factor (G-CSF) and the contamination by Ph+ cells and second, to quantify the amount of bcr-abl leukemia associated transcript by a quantitative assay during mobilization procedures, and post mobilization follow-up. Eight achieved a complete karyotypic remission before mobilization obtained with discontinuation of α-INF for few days and G-CSF at a dosage of 15μg/kg/day for 5-7 consecutive days. By quantitative-competitive polymerase chain reaction (QC-PCR) assay, all the leukaphereses and bone marrow samples during post mobilization follow up were studied to determine the amount of bcr-abl transcript. Karyotypic and molecular analysis on evaluable leukapheresis showed that all the harvests were Ph negative and bcr-abl positive: in seven cases the levels of bcr-abl transcript were higher or equal to the pre-apheresis status. In three out of four patients, who underwent more than one leukapheresis procedure, we noticed a decreasing amount of bcr-abl contamination from the first to the last apheresis. Our results suggest that in patients who achieved a complete or major cytogenetic conversion with α-INF, it is possible to obtain a sufficient amount of PBSC for autografting by leukapheresis following priming G-CSF therapy and that the amount of neoplastic transcript does not seem to increase.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalLeukemia and Lymphoma
Volume39
Issue number1-2
Publication statusPublished - 2000

Keywords

  • BCR/ABL
  • CML
  • PBSC
  • PCR-quantitative

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Martinelli, G., Montefusco, V., Amabile, M., Lemoli, R. M., Terragna, C., Testoni, N., Ottaviani, E., Rosti, G., De Vivo, A., Rizzi, S., Russo, D., Bregoli, M., & Tura, S. (2000). Quantitative evaluation of BCR-ABL amount of transcript post mobilization with G-CSF of peripheral blood stem cells from chronic myeloid leukemia patients in cytogenetic response. Leukemia and Lymphoma, 39(1-2), 113-120.