TY - JOUR
T1 - Quantitative flow cytometric analysis of the effects of cetirizine on the expression of ICAM-1/CD54 on primary cultured nasal cells
AU - Mincarini, M.
AU - Cagnoni, F.
AU - Canonica, Giorgio Walter
AU - Cordone, G.
AU - Sismondini, A.
AU - Semino, C.
AU - Pietra, G.
AU - Melioli, G.
PY - 2000
Y1 - 2000
N2 - An in vitro flow cytometric model has been developed to evaluate the effects of antiallergic drugs such as cetirizine (CTZ) on the expression of surface molecules on primary cultured normal cells. Quantitative analysis demonstrated that HLA class I and ICAM-1/CD54 molecules are present on both epithelial and stromal cells, and that their expression is strongly enhanced by treatment with interferon-gamma (IFN-γ). Nevertheless, the IFN-γ- mediated upregulation of ICAM-1/CD54 was inhibited by treatment with CTZ, demonstrating a direct effect on both cell types. This finding is particularly interesting because ICAM-1/CD54 is the main rhinovirus receptor, and rhinoviruses are the principal cause of asthma exacerbation in children. Thus, according to data derived from this in vitro model, CTZ should have an important role in the reduction of infectious exacerbation of asthma in atopic patients.
AB - An in vitro flow cytometric model has been developed to evaluate the effects of antiallergic drugs such as cetirizine (CTZ) on the expression of surface molecules on primary cultured normal cells. Quantitative analysis demonstrated that HLA class I and ICAM-1/CD54 molecules are present on both epithelial and stromal cells, and that their expression is strongly enhanced by treatment with interferon-gamma (IFN-γ). Nevertheless, the IFN-γ- mediated upregulation of ICAM-1/CD54 was inhibited by treatment with CTZ, demonstrating a direct effect on both cell types. This finding is particularly interesting because ICAM-1/CD54 is the main rhinovirus receptor, and rhinoviruses are the principal cause of asthma exacerbation in children. Thus, according to data derived from this in vitro model, CTZ should have an important role in the reduction of infectious exacerbation of asthma in atopic patients.
KW - CD54 (ICAM-1)
KW - Cetirizine
KW - Epithelial cells
KW - Fibroblast
KW - Flow cytometry
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U2 - 10.1034/j.1398-9995.2000.00213.x
DO - 10.1034/j.1398-9995.2000.00213.x
M3 - Article
C2 - 10753012
AN - SCOPUS:0034059489
VL - 55
SP - 226
EP - 231
JO - Allergy: European Journal of Allergy and Clinical Immunology
JF - Allergy: European Journal of Allergy and Clinical Immunology
SN - 0105-4538
IS - 3
ER -