Quantitative flow cytometry for the differential diagnosis of leukemic B-cell chronic lymphoproliferative disorders

Giovanni D'Arena, Pellegrino Musto, Nicola Cascavilla, Matteo Dell'Olio, Nicola Di Renzo, Mario Carotenuto

Research output: Contribution to journalArticle

Abstract

We have investigated whether the quantitative flow cytometry is an useful tool to better characterize B-cell chronic lymphoproliferative disorders (CLDs). Peripheral blood samples from 104 patients with leukemic B-cell disorders and 20 healthy donors were analyzed. Directly phycoerythrin-conjugated CD19, CD20, CD22, CD23, CD79b, and CD5 monoclonal antibodies (MoAbs) and QuantiBRITE pre-calibrated beads were used to calculate the number of antigen molecules per cell, expressed as antibody binding capacity (ABC). As compared to normal controls, in chronic lymphocytic leukemias (CLL) all MoAbs tested, with the exception of CD23 and CD5, showed lower ABC levels. In prolymphocytic leukemias (PL), CD5 and CD23 antigens were constantly absent while lower CD19 and CD22 ABC levels were observed. Hairy cell leukemias (HCL) displayed very high levels of CD20 and CD22. Of interest, splenic lymphomas with villous lymphocytes (SLVL) could be discriminated from HCL for higher CD79b and lower CD19 ABC values. Finally, higher CD20 levels were detected in follicular lymphomas (FL), whereas higher CD79b and CD5 levels characterized mantle cell lymphomas (MCL). Seven out of 61 CLL cases were defined as morphologically atypical. When compared with typical forms, lower levels of CD19 and CD23 and higher CD20 and CD22 ABC values were detected. However, we failed to demonstrate quantitative differences between atypical CLL and MCL. Our results suggest that quantitative flow cytometry may be a useful additional tool to better identify some types of B-cell CLDs. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalAmerican Journal of Hematology
Volume64
Issue number4
DOIs
Publication statusPublished - 2000

Fingerprint

Lymphoproliferative Disorders
Flow Cytometry
Differential Diagnosis
B-Lymphocytes
B-Cell Chronic Lymphocytic Leukemia
Antibodies
Mantle-Cell Lymphoma
Hairy Cell Leukemia
CD5 Antigens
Prolymphocytic Leukemia
Monoclonal Antibodies
Phycoerythrin
IgE Receptors
Follicular Lymphoma
Lymphoma
Tissue Donors
Lymphocytes
Antigens

Keywords

  • Chronic leukemias
  • Differential diagnosis
  • Flow cytometry
  • Quantitation

ASJC Scopus subject areas

  • Hematology

Cite this

Quantitative flow cytometry for the differential diagnosis of leukemic B-cell chronic lymphoproliferative disorders. / D'Arena, Giovanni; Musto, Pellegrino; Cascavilla, Nicola; Dell'Olio, Matteo; Renzo, Nicola Di; Carotenuto, Mario.

In: American Journal of Hematology, Vol. 64, No. 4, 2000, p. 275-281.

Research output: Contribution to journalArticle

D'Arena, Giovanni ; Musto, Pellegrino ; Cascavilla, Nicola ; Dell'Olio, Matteo ; Renzo, Nicola Di ; Carotenuto, Mario. / Quantitative flow cytometry for the differential diagnosis of leukemic B-cell chronic lymphoproliferative disorders. In: American Journal of Hematology. 2000 ; Vol. 64, No. 4. pp. 275-281.
@article{69f8591444d243928ad043da2be26263,
title = "Quantitative flow cytometry for the differential diagnosis of leukemic B-cell chronic lymphoproliferative disorders",
abstract = "We have investigated whether the quantitative flow cytometry is an useful tool to better characterize B-cell chronic lymphoproliferative disorders (CLDs). Peripheral blood samples from 104 patients with leukemic B-cell disorders and 20 healthy donors were analyzed. Directly phycoerythrin-conjugated CD19, CD20, CD22, CD23, CD79b, and CD5 monoclonal antibodies (MoAbs) and QuantiBRITE pre-calibrated beads were used to calculate the number of antigen molecules per cell, expressed as antibody binding capacity (ABC). As compared to normal controls, in chronic lymphocytic leukemias (CLL) all MoAbs tested, with the exception of CD23 and CD5, showed lower ABC levels. In prolymphocytic leukemias (PL), CD5 and CD23 antigens were constantly absent while lower CD19 and CD22 ABC levels were observed. Hairy cell leukemias (HCL) displayed very high levels of CD20 and CD22. Of interest, splenic lymphomas with villous lymphocytes (SLVL) could be discriminated from HCL for higher CD79b and lower CD19 ABC values. Finally, higher CD20 levels were detected in follicular lymphomas (FL), whereas higher CD79b and CD5 levels characterized mantle cell lymphomas (MCL). Seven out of 61 CLL cases were defined as morphologically atypical. When compared with typical forms, lower levels of CD19 and CD23 and higher CD20 and CD22 ABC values were detected. However, we failed to demonstrate quantitative differences between atypical CLL and MCL. Our results suggest that quantitative flow cytometry may be a useful additional tool to better identify some types of B-cell CLDs. (C) 2000 Wiley-Liss, Inc.",
keywords = "Chronic leukemias, Differential diagnosis, Flow cytometry, Quantitation",
author = "Giovanni D'Arena and Pellegrino Musto and Nicola Cascavilla and Matteo Dell'Olio and Renzo, {Nicola Di} and Mario Carotenuto",
year = "2000",
doi = "10.1002/1096-8652(200008)64:4<275::AID-AJH7>3.0.CO;2-Y",
language = "English",
volume = "64",
pages = "275--281",
journal = "American Journal of Hematology",
issn = "0361-8609",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Quantitative flow cytometry for the differential diagnosis of leukemic B-cell chronic lymphoproliferative disorders

AU - D'Arena, Giovanni

AU - Musto, Pellegrino

AU - Cascavilla, Nicola

AU - Dell'Olio, Matteo

AU - Renzo, Nicola Di

AU - Carotenuto, Mario

PY - 2000

Y1 - 2000

N2 - We have investigated whether the quantitative flow cytometry is an useful tool to better characterize B-cell chronic lymphoproliferative disorders (CLDs). Peripheral blood samples from 104 patients with leukemic B-cell disorders and 20 healthy donors were analyzed. Directly phycoerythrin-conjugated CD19, CD20, CD22, CD23, CD79b, and CD5 monoclonal antibodies (MoAbs) and QuantiBRITE pre-calibrated beads were used to calculate the number of antigen molecules per cell, expressed as antibody binding capacity (ABC). As compared to normal controls, in chronic lymphocytic leukemias (CLL) all MoAbs tested, with the exception of CD23 and CD5, showed lower ABC levels. In prolymphocytic leukemias (PL), CD5 and CD23 antigens were constantly absent while lower CD19 and CD22 ABC levels were observed. Hairy cell leukemias (HCL) displayed very high levels of CD20 and CD22. Of interest, splenic lymphomas with villous lymphocytes (SLVL) could be discriminated from HCL for higher CD79b and lower CD19 ABC values. Finally, higher CD20 levels were detected in follicular lymphomas (FL), whereas higher CD79b and CD5 levels characterized mantle cell lymphomas (MCL). Seven out of 61 CLL cases were defined as morphologically atypical. When compared with typical forms, lower levels of CD19 and CD23 and higher CD20 and CD22 ABC values were detected. However, we failed to demonstrate quantitative differences between atypical CLL and MCL. Our results suggest that quantitative flow cytometry may be a useful additional tool to better identify some types of B-cell CLDs. (C) 2000 Wiley-Liss, Inc.

AB - We have investigated whether the quantitative flow cytometry is an useful tool to better characterize B-cell chronic lymphoproliferative disorders (CLDs). Peripheral blood samples from 104 patients with leukemic B-cell disorders and 20 healthy donors were analyzed. Directly phycoerythrin-conjugated CD19, CD20, CD22, CD23, CD79b, and CD5 monoclonal antibodies (MoAbs) and QuantiBRITE pre-calibrated beads were used to calculate the number of antigen molecules per cell, expressed as antibody binding capacity (ABC). As compared to normal controls, in chronic lymphocytic leukemias (CLL) all MoAbs tested, with the exception of CD23 and CD5, showed lower ABC levels. In prolymphocytic leukemias (PL), CD5 and CD23 antigens were constantly absent while lower CD19 and CD22 ABC levels were observed. Hairy cell leukemias (HCL) displayed very high levels of CD20 and CD22. Of interest, splenic lymphomas with villous lymphocytes (SLVL) could be discriminated from HCL for higher CD79b and lower CD19 ABC values. Finally, higher CD20 levels were detected in follicular lymphomas (FL), whereas higher CD79b and CD5 levels characterized mantle cell lymphomas (MCL). Seven out of 61 CLL cases were defined as morphologically atypical. When compared with typical forms, lower levels of CD19 and CD23 and higher CD20 and CD22 ABC values were detected. However, we failed to demonstrate quantitative differences between atypical CLL and MCL. Our results suggest that quantitative flow cytometry may be a useful additional tool to better identify some types of B-cell CLDs. (C) 2000 Wiley-Liss, Inc.

KW - Chronic leukemias

KW - Differential diagnosis

KW - Flow cytometry

KW - Quantitation

UR - http://www.scopus.com/inward/record.url?scp=0033868231&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033868231&partnerID=8YFLogxK

U2 - 10.1002/1096-8652(200008)64:4<275::AID-AJH7>3.0.CO;2-Y

DO - 10.1002/1096-8652(200008)64:4<275::AID-AJH7>3.0.CO;2-Y

M3 - Article

C2 - 10911380

AN - SCOPUS:0033868231

VL - 64

SP - 275

EP - 281

JO - American Journal of Hematology

JF - American Journal of Hematology

SN - 0361-8609

IS - 4

ER -