Quantitative gas-liquid chromatographic determination of ftorafur and 5-fluorouracil in biological specimens

C. Pantarotto, R. Fanelli, S. Filippeschi, T. Facchinetti, F. Spreafico, M. Salmona

Research output: Contribution to journalArticlepeer-review

Abstract

A method developed for measuring the antitumor agent ftorafur and its biotransformation product 5-fluorouracil was applied to biological specimens. After extraction with ethylacetate, ftorafur, 5-fluorouracil, and the internal standard 2-methyl-4-hydroxy-6-chloromethylpyrimidine are converted to their chloromethyldimethylsilyl derivatives and assayed by glc, using either an electron-capture or a flame ionization detector. The minimum detectable amount is 100 pg/injection for ftorafur and 50 pg/injection for 5-fluorouracil employing electron-capture detection. Linearity was found up to microgram amounts of both substances, without any interference from endogenous substrates. Preliminary data are reported on the comparative serum kinetics of ftorafur and 5-fluorouracil in mice.

Original languageEnglish
Pages (from-to)232-238
Number of pages7
JournalAnalytical Biochemistry
Volume97
Issue number1
DOIs
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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