Quantitative identification of protein nitration sites

Giovanni Chiappetta, Claudia Corbo, Angelo Palmese, Gennaro Marino, Angela Amoresano

Research output: Contribution to journalArticlepeer-review

Abstract

Several labelling strategies have been developed targeting specific amino acid residues and/or PTMs. Methods specifically tailored for the qualitative and sometimes quantitative determination of PTMs have emerged. Many research groups have focused their attention towards o-nitrotyrosine residues, developing various methodologies for their identification, while direct quantification has remained elusive. So far the iTRAQ chemistry has been limited to primary amines. Here, we report a new strategy based on the use of iTRAQ reagents coupled to MS analysis for the selective labelling of o-nitrotyrosine residues. This method was proved to lead to the simultaneous localisation and quantification of nitration sites both in model proteins and in biological systems.

Original languageEnglish
Pages (from-to)1524-1537
Number of pages14
JournalProteomics
Volume9
Issue number6
DOIs
Publication statusPublished - Mar 2009

Keywords

  • ITRAQ
  • Mass spectrometry
  • Precursor ion
  • Protein nitration
  • Selective labelling

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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