Quantitative immunodetection of key elements of polyphosphoinositide signal transduction in osteoblasts from arthritic patients shows a direct correlation with cell proliferation

Nicoletta Zini, Gina Lisignoli, Liliana Solimando, Alberto Bavelloni, Aurelio Valmori, Sandra Cristino, Alberto Maria Martelli, Andrea Facchini, Nadir Mario Maraldi

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphoinositides play an essential role in diverse cellular functions such as cell proliferation, cytoskeletal regulation, intracellular vesicle trafficking, motility, cell metabolism and death. Alteration of these pathways is common to many diseases. In this study, we show that osteoblasts from patients affected by osteoarthritis (OA) and by rheumatoid arthritis (RA) present a decreased cell proliferation and a reduced expression of the key elements of polyphosphoinositide signal transduction such as phosphatidylinositol-3-kinase (PI 3K), phospholipase C γ1 (PLCγ1), and protein kinase C ζ (PKCζ) compared to the post-traumatic (PT) patients. Our results suggest that a correlation may exist between the reduced osteoblast proliferation observed in OA and RA patients and the lowered expression of PI 3K, PLCγ1, and PKCζ enzymes. The reduced proliferation rate of osteoblasts in response to these signal transduction effectors could counteract the evolution of arthritic disease.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalHistochemistry and Cell Biology
Volume124
Issue number2
DOIs
Publication statusPublished - Aug 2005

Keywords

  • Osteoarthritis
  • Osteoblasts
  • Rheumatoid arthritis
  • Signal transduction

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

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