Quantitative immunohistochemistry of glucose transport protein (glut3) expression in the rat hippocampus during aging

Patrizia Fattoretti, Carlo Bertoni-Freddari, Giuseppina Di Stefano, Tiziana Casoli, Natascia Gracciotti, Moreno Solazzi, Pierluigi Pompei

Research output: Contribution to journalArticle

Abstract

Immunohistochemistry of Glut3 (45 kD), an integral membrane peptide mediating the transport of glucose in neurons, was carried out in the hippocampus of 3- and 28-month-old rats to assess the effect of age on energy metabolism. Free-floating sections of fixed-frozen hippocampi were processed for quantitative immunohistochemistry of Glut3. A rabbit affinity-purified antibody identified Glut3 immunoreactivity. Glut3 staining was intense in neuropil, axons, and dendrites, whereas nerve cell bodies were unstained. With aging, Glut3 reactivity was significantly decreased in the inner molecular layer of the hippocampal dentate gyrus (-46%) and the mossy fibers of the CA3 sector (-34%), whereas the stratum radiatum of CA1 did not show any difference due to age. These data document an age-dependent decrease in Glut3 expression in discrete areas of rat hippocampus. Glut3 constitutes the predominant glucose transporter in neurons and is found abundantly in regions with high synaptic density characterized by frequent bursts of function-adequate metabolic activity. Our findings therefore lend further support to the critical role of an impaired metabolism in age-related brain dysfunctions and disease.

Original languageEnglish
Pages (from-to)671-672
Number of pages2
JournalJournal of Histochemistry and Cytochemistry
Volume49
Issue number5
Publication statusPublished - 2001

Keywords

  • Aging
  • Glut3
  • Hippocampus
  • Metabolism

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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