Quantitative indirect immunofluorescence as an alternative to Western blot for the diagnosis and follow-up of pediatric AIDS

S. Calarota, D. Liberatore, R. Rabinovich, O. Libonatti, L. M. Peralta

Research output: Contribution to journalArticlepeer-review

Abstract

The main objective of this work was to evaluate quantitative indirect immunofluorescence assay (IFA) in order to replace Western blot (WB) for the diagnosis and follow-up of children born to human immunodeficiency virus type 1 (HIV-1)-infected mothers. A total of 102 sera were obtained from 23 infants. The clinical status was determined according to the Centers for Disease Control and Prevention (CDC) classification: 8 infants were P2 and 15 were noninfected. Immunoglobulin G (IgG) titers were determined by IFA, and commercial WB assay was performed in all samples. In all infected children, IgG titers increased with the appearance of new bands or an increase in the intensity of previously reactive bands in the WB, which corresponded to the worsening of the clinical status. The 15 noninfected children presented decreasing IgG titers corresponding to the loss of WB reactivity. In 4 of these infants negative IFA results appeared 6 months earlier on average than negative WB results. Our data suggest that IFA is a cost-effective alternative to WB for the follow-up and diagnosis of pediatric acquired immunodeficiency syndrome (AIDS).

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalPediatric AIDS and HIV Infection
Volume5
Issue number2
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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