Quantitative multiplexed C-reactive protein mass spectrometric immunoassay

Urban A. Kiernan, Riccardo Addobbati, Dobrin Nedelkov, Randall W. Nelson

Research output: Contribution to journalArticlepeer-review

Abstract

Reported in this work is the development and application of a high sensitivity mass spectrometric immunoassay for the quantitative analysis of C-reactive protein from human plasma. Multiplexed affinity retrieval devices and methodology were developed to simultaneously target retinol binding protein, C-reactive protein, serum amyloid P component, as well as an added exogenous internal reference standard (staphylococcal enterotoxin B) for subsequent MALDI-TOF MS analysis. This approach allows for semiquantitative analysis of both retinol binding protein and serum amyloid P component while performing absolute quantitative measurements of C-reactive protein. The ability to qualitatively differentiate between all three human proteins and their associated variants is also maintained. Standard curve, QC, and human plasma samples were analyzed in a high throughput manner, which performed with a CV <15%. The resultant human plasma sample C-reactive protein quantitative measurements were then compared to those achieved with a high sensitivity latex immunoturbidimetric assay.

Original languageEnglish
Pages (from-to)1682-1687
Number of pages6
JournalJournal of Proteome Research
Volume5
Issue number7
DOIs
Publication statusPublished - Jul 2006

Keywords

  • C-reactive protein
  • Human plasma
  • Immunoassay
  • Mass spectrometry
  • Multiplexing
  • Quantitation

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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