Quantitative pharmacohistochemistry of acetylcholinesterase in neostriatum of inbred strains of mice

Carmela Iacopino, Maria Concetta Altavista, Stefano Gozzo, Alberto Albanese

Research output: Contribution to journalArticle

Abstract

A quantitative pharmacohistochemical technique has been used in the present study to assay acetylcholinesterase (AChE) activity in the neostriatum of C57BL/6 and DBA/2 mice. This technique permits the measurement of enzyme activity into microscopically defined compartments and is suitable for the study of striatal AChE-containing, putatively cholinergic, neurons. Microphotometric measurements have been performed in the cytoplasm of AChE-containing perikarya and in the striatal matrix: in both compartments, AChE activity was significantly higher in DBA/2 than in C57BL/6 mice. The present data show that AChE quantitative pharmacohistochemistry is suitable for studying the enzyme activity in nervous tissue and, particularly, in the cytoplasm of individual AChE-containing neurons. In addition, interstrain comparison indicates the presence of a genetically determined higher AChE content in striatal neurons of the DBA/2 strain.

Original languageEnglish
Pages (from-to)402-408
Number of pages7
JournalBrain Research
Volume374
Issue number2
DOIs
Publication statusPublished - May 28 1986

Keywords

  • acetylcholinesterase
  • animal strain
  • basal ganglion
  • cholinergic system
  • genetic study
  • histochemistry
  • microphotometry

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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