TY - JOUR
T1 - Quantitative profiling of 6-ketoprostaglandin F1α, 2,3-dinor-6-ketoprostaglandin F1α, thromboxane B2 and 2,3-dinor-thromboxane B2 in human and rat urine by immunoaffinity extraction with gas chromatography-mass spectrometry
AU - Chiabrando, Chiara
AU - Pinciroli, Vittorio
AU - Campoleoni, Andrea
AU - Benigni, Ariela
AU - Piccinelli, Antonella
AU - Fanelli, Roberto
PY - 1989
Y1 - 1989
N2 - A rapid and simple method based on immunoaffinity extraction, stable isotope dilution and gas chromatography-mass spectrometry has been developed for profiling urinary metabolites of prostacyclin and thromboxane. 6-Ketoprostaglandin F1α (6-keto-PGF1α), 2,3-dinor-6-ketoprostaglandin F1α (2,3-dinor-6-keto-PGF1α), thromboxane B2 (TXB2) and 2,3-dinor-thromboxane B2 (2,3-dinor-TXB2) were quantitatively extracted from human or rat urine spiked with deuterated internal standards using mixed-bed columns containing immobilized anti-6-keto-PGF1α and anti-TXB2 antibodies (cross-reacting with 2,3-dinor-6-keto-PGF1α and 2,3-dinor-TXB2, respectively). The extract was directly derivatized to form pentafluorobenzyl ester, methyloxime, trimethylsilyl ether derivatives. Quantitation was performed by stable isotope dilution assay and high-resolution gas chromatography-negative ion chemical ionization mass spectrometry, by monitoring the carboxylate anions (M - 181) of the derivatized metabolites. The method was applied to evaluate the urinary excretion of 6-keto PGF1α, 2,3-dinor-6-keto-PGF1α, TXB2 and 2,3-dinor-TXB2 in humans and rats. Results were in accordance with previously reported data obtained by other methods. Novel data on the urinary excretion of 2,3-dinor-6-keto-PGF1α in rats under basal conditions are presented. This sensitive and selective method represents a significant advance in terms of rapidity and simplicity over other immunoaffinity-gas chromatography-mass spectrometry methods for measuring single prostanoids, such as 6-keto-PGF1α or TXB2, since it allows profiling of a group of metabolites whose balance is important in several physiopathological conditions.
AB - A rapid and simple method based on immunoaffinity extraction, stable isotope dilution and gas chromatography-mass spectrometry has been developed for profiling urinary metabolites of prostacyclin and thromboxane. 6-Ketoprostaglandin F1α (6-keto-PGF1α), 2,3-dinor-6-ketoprostaglandin F1α (2,3-dinor-6-keto-PGF1α), thromboxane B2 (TXB2) and 2,3-dinor-thromboxane B2 (2,3-dinor-TXB2) were quantitatively extracted from human or rat urine spiked with deuterated internal standards using mixed-bed columns containing immobilized anti-6-keto-PGF1α and anti-TXB2 antibodies (cross-reacting with 2,3-dinor-6-keto-PGF1α and 2,3-dinor-TXB2, respectively). The extract was directly derivatized to form pentafluorobenzyl ester, methyloxime, trimethylsilyl ether derivatives. Quantitation was performed by stable isotope dilution assay and high-resolution gas chromatography-negative ion chemical ionization mass spectrometry, by monitoring the carboxylate anions (M - 181) of the derivatized metabolites. The method was applied to evaluate the urinary excretion of 6-keto PGF1α, 2,3-dinor-6-keto-PGF1α, TXB2 and 2,3-dinor-TXB2 in humans and rats. Results were in accordance with previously reported data obtained by other methods. Novel data on the urinary excretion of 2,3-dinor-6-keto-PGF1α in rats under basal conditions are presented. This sensitive and selective method represents a significant advance in terms of rapidity and simplicity over other immunoaffinity-gas chromatography-mass spectrometry methods for measuring single prostanoids, such as 6-keto-PGF1α or TXB2, since it allows profiling of a group of metabolites whose balance is important in several physiopathological conditions.
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U2 - 10.1016/S0378-4347(00)82604-3
DO - 10.1016/S0378-4347(00)82604-3
M3 - Article
C2 - 2613794
AN - SCOPUS:0024460880
VL - 495
SP - 1
EP - 11
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - C
ER -