Quantitative profiling of prostaglandins and thromboxane by high-resolution gas chromatography-selected-ion monitoring

C. Chiabrando, A. Noseda, M. N. Castagnoli, M. Romano, R. Fanelli

Research output: Contribution to journalArticlepeer-review

Abstract

The development and biological application of a rapid method for quatitative profiling of prostaglandins and thromboxane using high-resolution gas chromatography (HRGC) coupled with mass spectrometry in the selected-ion monitoring technique (SIM) are described. The method is based on the single-step extraction of prostaglandins from biological samples on C18 reversed-phase cartridges after addition of deuterated analogues as internal standards, followed by derivatization of functional groups and final analysis by HRGC-SIM with wall-coated open tubular persilanized capillary columns. Biological applications include the determination of endogenous arachidonic acid cascade profiles in rat tissue homogenates and thromboxane synthetase inhibition studies in human serum.

Original languageEnglish
Pages (from-to)581-586
Number of pages6
JournalJournal of Chromatography A
Volume279
Issue numberC
DOIs
Publication statusPublished - Nov 25 1983

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

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