Quantitative proteomics reveals novel therapeutic and diagnostic markers in hypertension

Vittoria Matafora, Laura Zagato, Mara Ferrandi, Isabella Molinari, Gianpaolo Zerbini, Nunzia Casamassima, Chiara Lanzani, Simona Delli Carpini, Francesco Trepiccione, Paolo Manunta, Angela Bachi, Giovambattista Capasso

Research output: Contribution to journalArticlepeer-review

Abstract

Hypertension is a prevalent disorder in the world representing one of the major risk factors for heart attack and stroke. These risks are increased in salt sensitive individuals. Hypertension and salt sensitivity are complex phenotypes whose pathophysiology remains poorly understood and, remarkably, salt sensitivity is still laborious to diagnose.Here we present a urinary proteomic study specifically designed to identify urinary proteins relevant for the pathogenesis of hypertension and salt sensitivity. Despite previous studies that underlined the association of UMOD gene variants with hypertension, this work provides novel evidence showing different uromodulin protein level in the urine of hypertensive patients compared to healthy individuals. Notably, we also show that patients with higher level of uromodulin are homozygous for UMOD risk variant and display a decreased level of salt excretion, highlighting the essential role of UMOD in the regulation of salt reabsorption in hypertension. Additionally, we found that urinary nephrin 1, a marker of glomerular slit diaphragm, may predict a salt sensitive phenotype and positively correlate with increased albuminuria associated with this type of hypertension.

Original languageEnglish
Pages (from-to)79-87
Number of pages9
JournalBBA Clinical
Volume2
DOIs
Publication statusPublished - Dec 1 2014

Keywords

  • Glomerular injury
  • Nephrinuria
  • Quantitative proteomics
  • Salt homeostasis
  • Salt sensitive hypertension
  • Urinary biomarker
  • Uromodulin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Physiology (medical)
  • Molecular Medicine

Fingerprint Dive into the research topics of 'Quantitative proteomics reveals novel therapeutic and diagnostic markers in hypertension'. Together they form a unique fingerprint.

Cite this