Quantitative Real-Time PCR in Cancer Research

Simone Mocellin, Carlo R. Rossi, Francesco M. Marincola

Research output: Contribution to journalArticle

Abstract

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstrumentation for gene level measurement. Several applications have been already implemented in the field of cancer research, and others are being validated, showing that this molecular biology tool can provide both researchers and clinicians with precious information concerning the behaviour of tumors. The knowledge of the biochemical principles underlying this biotechnology can be of great value to correctly interpret qrt-PCR data.

Original languageEnglish
Pages (from-to)301-313
Number of pages13
JournalArchivum Immunologiae et Therapiae Experimentalis
Volume51
Issue number5
Publication statusPublished - 2003

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Real-Time Polymerase Chain Reaction
Research
Neoplasms
Human Genome Project
Biotechnology
Genes
Molecular Biology
Research Personnel
Technology
Gene Expression

Keywords

  • Cancer research
  • Quantitative PCR

ASJC Scopus subject areas

  • Immunology

Cite this

Quantitative Real-Time PCR in Cancer Research. / Mocellin, Simone; Rossi, Carlo R.; Marincola, Francesco M.

In: Archivum Immunologiae et Therapiae Experimentalis, Vol. 51, No. 5, 2003, p. 301-313.

Research output: Contribution to journalArticle

Mocellin, Simone ; Rossi, Carlo R. ; Marincola, Francesco M. / Quantitative Real-Time PCR in Cancer Research. In: Archivum Immunologiae et Therapiae Experimentalis. 2003 ; Vol. 51, No. 5. pp. 301-313.
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