Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues

B. Barbieri; R. Abbruzzi; A. Benigni; M. Rizzardini; M. G. Donelli; S. Garattini; M. Salmona

doi:10.1016/S0378-4347(00)81463-2

Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues

B. Barbieri, R. Abbruzzi, A. Benigni, M. Rizzardini, M. G. Donelli, S. Garattini, M. Salmona

Istituto di Ricerche Farmacologiche "Mario Negri"

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Abstract

A thin-layer chromatographic method has been developed for the detection and measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and its major metabolite trifluoroacetyladriamycin (AD 41). The procedure gives satisfactory linearity over a large range of concentrations. The coefficient of variability is about 10% over the entire range of usable concentrations, giving good reproducibility; sensitivity is 25 ng for both AD 32 and AD 41. Analysis is specific for AD 32 and AD 41 since adriamycin or more polar metabolites can be differentiated. Recovery is high (85-90%) and the method is simple and economical to use. Pharmacokinetics of AD 32 and AD 41 are reported in blood and some tissues of mice bearing Lewis Lung carcinoma.

Original language	English
Pages (from-to)	195-200
Number of pages	6
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	163
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(00)81463-2
Publication status	Published - Jun 11 1979

ASJC Scopus subject areas

Chemistry(all)
Clinical Biochemistry
Molecular Medicine

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10.1016/S0378-4347(00)81463-2

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Barbieri, B., Abbruzzi, R., Benigni, A., Rizzardini, M., Donelli, M. G., Garattini, S., & Salmona, M. (1979). Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues. Journal of Chromatography B: Biomedical Sciences and Applications, 163(2), 195-200. https://doi.org/10.1016/S0378-4347(00)81463-2

Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues. / Barbieri, B.; Abbruzzi, R.; Benigni, A.; Rizzardini, M.; Donelli, M. G.; Garattini, S.; Salmona, M.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 163, No. 2, 11.06.1979, p. 195-200.

Research output: Contribution to journal › Article › peer-review

Barbieri, B, Abbruzzi, R, Benigni, A, Rizzardini, M, Donelli, MG, Garattini, S & Salmona, M 1979, 'Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 163, no. 2, pp. 195-200. https://doi.org/10.1016/S0378-4347(00)81463-2

Barbieri B, Abbruzzi R, Benigni A, Rizzardini M, Donelli MG, Garattini S et al. Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues. Journal of Chromatography B: Biomedical Sciences and Applications. 1979 Jun 11;163(2):195-200. https://doi.org/10.1016/S0378-4347(00)81463-2

Barbieri, B. ; Abbruzzi, R. ; Benigni, A. ; Rizzardini, M. ; Donelli, M. G. ; Garattini, S. ; Salmona, M. / Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1979 ; Vol. 163, No. 2. pp. 195-200.

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abstract = "A thin-layer chromatographic method has been developed for the detection and measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and its major metabolite trifluoroacetyladriamycin (AD 41). The procedure gives satisfactory linearity over a large range of concentrations. The coefficient of variability is about 10% over the entire range of usable concentrations, giving good reproducibility; sensitivity is 25 ng for both AD 32 and AD 41. Analysis is specific for AD 32 and AD 41 since adriamycin or more polar metabolites can be differentiated. Recovery is high (85-90%) and the method is simple and economical to use. Pharmacokinetics of AD 32 and AD 41 are reported in blood and some tissues of mice bearing Lewis Lung carcinoma.",

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AU - Benigni, A.

AU - Rizzardini, M.

AU - Donelli, M. G.

AU - Garattini, S.

AU - Salmona, M.

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AB - A thin-layer chromatographic method has been developed for the detection and measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and its major metabolite trifluoroacetyladriamycin (AD 41). The procedure gives satisfactory linearity over a large range of concentrations. The coefficient of variability is about 10% over the entire range of usable concentrations, giving good reproducibility; sensitivity is 25 ng for both AD 32 and AD 41. Analysis is specific for AD 32 and AD 41 since adriamycin or more polar metabolites can be differentiated. Recovery is high (85-90%) and the method is simple and economical to use. Pharmacokinetics of AD 32 and AD 41 are reported in blood and some tissues of mice bearing Lewis Lung carcinoma.

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Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues

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