Abstract
A thin-layer chromatographic method has been developed for the detection and measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and its major metabolite trifluoroacetyladriamycin (AD 41). The procedure gives satisfactory linearity over a large range of concentrations. The coefficient of variability is about 10% over the entire range of usable concentrations, giving good reproducibility; sensitivity is 25 ng for both AD 32 and AD 41. Analysis is specific for AD 32 and AD 41 since adriamycin or more polar metabolites can be differentiated. Recovery is high (85-90%) and the method is simple and economical to use. Pharmacokinetics of AD 32 and AD 41 are reported in blood and some tissues of mice bearing Lewis Lung carcinoma.
| Original language | English |
|---|---|
| Pages (from-to) | 195-200 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 163 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 11 1979 |
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ASJC Scopus subject areas
- Chemistry(all)
- Clinical Biochemistry
- Molecular Medicine
Cite this
Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues. / Barbieri, B.; Abbruzzi, R.; Benigni, A.; Rizzardini, M.; Donelli, M. G.; Garattini, S.; Salmona, M.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 163, No. 2, 11.06.1979, p. 195-200.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Quantitative thin-layer chromatographic measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues
AU - Barbieri, B.
AU - Abbruzzi, R.
AU - Benigni, A.
AU - Rizzardini, M.
AU - Donelli, M. G.
AU - Garattini, S.
AU - Salmona, M.
PY - 1979/6/11
Y1 - 1979/6/11
N2 - A thin-layer chromatographic method has been developed for the detection and measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and its major metabolite trifluoroacetyladriamycin (AD 41). The procedure gives satisfactory linearity over a large range of concentrations. The coefficient of variability is about 10% over the entire range of usable concentrations, giving good reproducibility; sensitivity is 25 ng for both AD 32 and AD 41. Analysis is specific for AD 32 and AD 41 since adriamycin or more polar metabolites can be differentiated. Recovery is high (85-90%) and the method is simple and economical to use. Pharmacokinetics of AD 32 and AD 41 are reported in blood and some tissues of mice bearing Lewis Lung carcinoma.
AB - A thin-layer chromatographic method has been developed for the detection and measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and its major metabolite trifluoroacetyladriamycin (AD 41). The procedure gives satisfactory linearity over a large range of concentrations. The coefficient of variability is about 10% over the entire range of usable concentrations, giving good reproducibility; sensitivity is 25 ng for both AD 32 and AD 41. Analysis is specific for AD 32 and AD 41 since adriamycin or more polar metabolites can be differentiated. Recovery is high (85-90%) and the method is simple and economical to use. Pharmacokinetics of AD 32 and AD 41 are reported in blood and some tissues of mice bearing Lewis Lung carcinoma.
UR - http://www.scopus.com/inward/record.url?scp=0018776577&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018776577&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)81463-2
DO - 10.1016/S0378-4347(00)81463-2
M3 - Article
C2 - 541371
AN - SCOPUS:0018776577
VL - 163
SP - 195
EP - 200
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 2
ER -