Quantitatively different red cell/nucleated cell chimerism in patients with long-term, persistent hematopoietic mixed chimerism after bone marrow transplantation for thalassemia major or sickle cell disease

Marco Andreani, Manuela Testi, Javid Gaziev, Rossella Condello, Andrea Bontadini, Pier Luigi Tazzari, Francesca Ricci, Lidia de Felice, Francesca Agostini, Daniela Fraboni, Giuliana Ferrari, Mariarosa Battarra, Maria Troiano, Pietro Sodani, Guido Lucarelli

Research output: Contribution to journalArticle

Abstract

Background Persistent mixed chimerism represents a state in which recipient and donor cells stably co-exist after hematopoietic stem cell transplantation. However, since in most of the studies reported in literature the engraftment state was observed in the nucleated cells, in this study we determined the donor origin of the mature erythrocytes of patients with persistent mixed chimerism after transplantation for hemoglobinopathies. Results were compared with the engraftment state observed in singly picked out burst-forming unit - erythroid colonies and in the nucleated cells collected from the peripheral blood and from the bone marrow. Design and Methods The donor origin of the erythrocytes was determined analyzing differences on the surface antigens of the erythrocyte suspension after incubation with anti-ABO and/or anti-C, -c, -D, -E and -e monoclonal antibodies by a flow cytometer. Analysis of short tandem repeats was used to determine the donor origin of nucleated cells and burst-forming unit - erythroid colonies singly picked out after 14 days of incubation. Results The proportions of donor-derived nucleated cells in four transplanted patients affected by hemoglobinopathies were 71%, 46%, 15% and 25% at day 1364, 1385, 1314 and 932, respectively. Similar results were obtained for the erythroid precursors, analyzing the donor/recipient origin of the burst-forming unit - erythroid colonies. In contrast, on the same days of observation, the proportions of donor-derived erythrocytes in the four patients with persistent mixed chimerism were 100%, 100%, 73% and 90%. Conclusions Our results showed that most of the erythrocytes present in four long-term transplanted patients affected by hemoglobinopathies and characterized by the presence of few donor engrafted nucleated cells were of donor origin. The indication that small proportions of donor engrafted cells might be sufficient for clinical control of the disease in patients affected by hemoglobinopathies is relevant, although the biological mechanisms underlying these observations need further investigation.

Original languageEnglish
Pages (from-to)128-133
Number of pages6
JournalHaematologica
Volume96
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Chimerism
beta-Thalassemia
Sickle Cell Anemia
Bone Marrow Transplantation
Tissue Donors
Hemoglobinopathies
Erythrocytes
Erythroid Precursor Cells
Hematopoietic Stem Cell Transplantation
Surface Antigens
Microsatellite Repeats
Suspensions
Transplantation
Bone Marrow
Monoclonal Antibodies
Observation

Keywords

  • Hematopoietic stem cell transplantation
  • Hemoglobinopathies
  • Persistent mixed chimerism

ASJC Scopus subject areas

  • Hematology

Cite this

Quantitatively different red cell/nucleated cell chimerism in patients with long-term, persistent hematopoietic mixed chimerism after bone marrow transplantation for thalassemia major or sickle cell disease. / Andreani, Marco; Testi, Manuela; Gaziev, Javid; Condello, Rossella; Bontadini, Andrea; Tazzari, Pier Luigi; Ricci, Francesca; de Felice, Lidia; Agostini, Francesca; Fraboni, Daniela; Ferrari, Giuliana; Battarra, Mariarosa; Troiano, Maria; Sodani, Pietro; Lucarelli, Guido.

In: Haematologica, Vol. 96, No. 1, 01.2011, p. 128-133.

Research output: Contribution to journalArticle

Andreani, M, Testi, M, Gaziev, J, Condello, R, Bontadini, A, Tazzari, PL, Ricci, F, de Felice, L, Agostini, F, Fraboni, D, Ferrari, G, Battarra, M, Troiano, M, Sodani, P & Lucarelli, G 2011, 'Quantitatively different red cell/nucleated cell chimerism in patients with long-term, persistent hematopoietic mixed chimerism after bone marrow transplantation for thalassemia major or sickle cell disease', Haematologica, vol. 96, no. 1, pp. 128-133. https://doi.org/10.3324/haematol.2010.031013
Andreani, Marco ; Testi, Manuela ; Gaziev, Javid ; Condello, Rossella ; Bontadini, Andrea ; Tazzari, Pier Luigi ; Ricci, Francesca ; de Felice, Lidia ; Agostini, Francesca ; Fraboni, Daniela ; Ferrari, Giuliana ; Battarra, Mariarosa ; Troiano, Maria ; Sodani, Pietro ; Lucarelli, Guido. / Quantitatively different red cell/nucleated cell chimerism in patients with long-term, persistent hematopoietic mixed chimerism after bone marrow transplantation for thalassemia major or sickle cell disease. In: Haematologica. 2011 ; Vol. 96, No. 1. pp. 128-133.
@article{54e85fd744a3433c92756d447588e629,
title = "Quantitatively different red cell/nucleated cell chimerism in patients with long-term, persistent hematopoietic mixed chimerism after bone marrow transplantation for thalassemia major or sickle cell disease",
abstract = "Background Persistent mixed chimerism represents a state in which recipient and donor cells stably co-exist after hematopoietic stem cell transplantation. However, since in most of the studies reported in literature the engraftment state was observed in the nucleated cells, in this study we determined the donor origin of the mature erythrocytes of patients with persistent mixed chimerism after transplantation for hemoglobinopathies. Results were compared with the engraftment state observed in singly picked out burst-forming unit - erythroid colonies and in the nucleated cells collected from the peripheral blood and from the bone marrow. Design and Methods The donor origin of the erythrocytes was determined analyzing differences on the surface antigens of the erythrocyte suspension after incubation with anti-ABO and/or anti-C, -c, -D, -E and -e monoclonal antibodies by a flow cytometer. Analysis of short tandem repeats was used to determine the donor origin of nucleated cells and burst-forming unit - erythroid colonies singly picked out after 14 days of incubation. Results The proportions of donor-derived nucleated cells in four transplanted patients affected by hemoglobinopathies were 71{\%}, 46{\%}, 15{\%} and 25{\%} at day 1364, 1385, 1314 and 932, respectively. Similar results were obtained for the erythroid precursors, analyzing the donor/recipient origin of the burst-forming unit - erythroid colonies. In contrast, on the same days of observation, the proportions of donor-derived erythrocytes in the four patients with persistent mixed chimerism were 100{\%}, 100{\%}, 73{\%} and 90{\%}. Conclusions Our results showed that most of the erythrocytes present in four long-term transplanted patients affected by hemoglobinopathies and characterized by the presence of few donor engrafted nucleated cells were of donor origin. The indication that small proportions of donor engrafted cells might be sufficient for clinical control of the disease in patients affected by hemoglobinopathies is relevant, although the biological mechanisms underlying these observations need further investigation.",
keywords = "Hematopoietic stem cell transplantation, Hemoglobinopathies, Persistent mixed chimerism",
author = "Marco Andreani and Manuela Testi and Javid Gaziev and Rossella Condello and Andrea Bontadini and Tazzari, {Pier Luigi} and Francesca Ricci and {de Felice}, Lidia and Francesca Agostini and Daniela Fraboni and Giuliana Ferrari and Mariarosa Battarra and Maria Troiano and Pietro Sodani and Guido Lucarelli",
year = "2011",
month = "1",
doi = "10.3324/haematol.2010.031013",
language = "English",
volume = "96",
pages = "128--133",
journal = "Haematologica",
issn = "0390-6078",
publisher = "NLM (Medline)",
number = "1",

}

TY - JOUR

T1 - Quantitatively different red cell/nucleated cell chimerism in patients with long-term, persistent hematopoietic mixed chimerism after bone marrow transplantation for thalassemia major or sickle cell disease

AU - Andreani, Marco

AU - Testi, Manuela

AU - Gaziev, Javid

AU - Condello, Rossella

AU - Bontadini, Andrea

AU - Tazzari, Pier Luigi

AU - Ricci, Francesca

AU - de Felice, Lidia

AU - Agostini, Francesca

AU - Fraboni, Daniela

AU - Ferrari, Giuliana

AU - Battarra, Mariarosa

AU - Troiano, Maria

AU - Sodani, Pietro

AU - Lucarelli, Guido

PY - 2011/1

Y1 - 2011/1

N2 - Background Persistent mixed chimerism represents a state in which recipient and donor cells stably co-exist after hematopoietic stem cell transplantation. However, since in most of the studies reported in literature the engraftment state was observed in the nucleated cells, in this study we determined the donor origin of the mature erythrocytes of patients with persistent mixed chimerism after transplantation for hemoglobinopathies. Results were compared with the engraftment state observed in singly picked out burst-forming unit - erythroid colonies and in the nucleated cells collected from the peripheral blood and from the bone marrow. Design and Methods The donor origin of the erythrocytes was determined analyzing differences on the surface antigens of the erythrocyte suspension after incubation with anti-ABO and/or anti-C, -c, -D, -E and -e monoclonal antibodies by a flow cytometer. Analysis of short tandem repeats was used to determine the donor origin of nucleated cells and burst-forming unit - erythroid colonies singly picked out after 14 days of incubation. Results The proportions of donor-derived nucleated cells in four transplanted patients affected by hemoglobinopathies were 71%, 46%, 15% and 25% at day 1364, 1385, 1314 and 932, respectively. Similar results were obtained for the erythroid precursors, analyzing the donor/recipient origin of the burst-forming unit - erythroid colonies. In contrast, on the same days of observation, the proportions of donor-derived erythrocytes in the four patients with persistent mixed chimerism were 100%, 100%, 73% and 90%. Conclusions Our results showed that most of the erythrocytes present in four long-term transplanted patients affected by hemoglobinopathies and characterized by the presence of few donor engrafted nucleated cells were of donor origin. The indication that small proportions of donor engrafted cells might be sufficient for clinical control of the disease in patients affected by hemoglobinopathies is relevant, although the biological mechanisms underlying these observations need further investigation.

AB - Background Persistent mixed chimerism represents a state in which recipient and donor cells stably co-exist after hematopoietic stem cell transplantation. However, since in most of the studies reported in literature the engraftment state was observed in the nucleated cells, in this study we determined the donor origin of the mature erythrocytes of patients with persistent mixed chimerism after transplantation for hemoglobinopathies. Results were compared with the engraftment state observed in singly picked out burst-forming unit - erythroid colonies and in the nucleated cells collected from the peripheral blood and from the bone marrow. Design and Methods The donor origin of the erythrocytes was determined analyzing differences on the surface antigens of the erythrocyte suspension after incubation with anti-ABO and/or anti-C, -c, -D, -E and -e monoclonal antibodies by a flow cytometer. Analysis of short tandem repeats was used to determine the donor origin of nucleated cells and burst-forming unit - erythroid colonies singly picked out after 14 days of incubation. Results The proportions of donor-derived nucleated cells in four transplanted patients affected by hemoglobinopathies were 71%, 46%, 15% and 25% at day 1364, 1385, 1314 and 932, respectively. Similar results were obtained for the erythroid precursors, analyzing the donor/recipient origin of the burst-forming unit - erythroid colonies. In contrast, on the same days of observation, the proportions of donor-derived erythrocytes in the four patients with persistent mixed chimerism were 100%, 100%, 73% and 90%. Conclusions Our results showed that most of the erythrocytes present in four long-term transplanted patients affected by hemoglobinopathies and characterized by the presence of few donor engrafted nucleated cells were of donor origin. The indication that small proportions of donor engrafted cells might be sufficient for clinical control of the disease in patients affected by hemoglobinopathies is relevant, although the biological mechanisms underlying these observations need further investigation.

KW - Hematopoietic stem cell transplantation

KW - Hemoglobinopathies

KW - Persistent mixed chimerism

UR - http://www.scopus.com/inward/record.url?scp=78650991791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650991791&partnerID=8YFLogxK

U2 - 10.3324/haematol.2010.031013

DO - 10.3324/haematol.2010.031013

M3 - Article

C2 - 20935000

AN - SCOPUS:78650991791

VL - 96

SP - 128

EP - 133

JO - Haematologica

JF - Haematologica

SN - 0390-6078

IS - 1

ER -